Interviewee: Stephen Fodor.
Stephen Fodor talks about the photolithographic technique used to synthesize pieces of DNA on the surface of GeneChips™.
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GeneChips™, step by step)
The way we make these DNA chips is that we actually build the DNA step by step at each location on one of these chips. In a process known as photolithography we excite with light a certain region on the chip and that prepares that particular site for chemical reaction. One of the four building blocks, A, C, G or T are applied and wherever we shine the light, that building block will stick and grow. And so we go through a multiple number of steps in this way, over literally millions of different sites on a large piece of glass, and what we get are literally millions of different precise locations on the chip, each with their own different strand of DNA on it.
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Igor Dawid and Thomas Sargent explain how they developed subtractive mRNA hybrization to find genes expressed by different cell types. Pat Brown and Steve Fodor show how genomes can be screened with DNA arrays and GeneChipsï¿½