anti his antibody for western blotting

Hi
I am doing some western blotting for an intracellular his tagged recombinant protein in CHO cell lysate, I tried anti his HRP from roche and anti penta his from qiagen followed by conjugated second antibody. with anti his HRP I didnt get any band even in 200 microgram lysate but giagen antibody in its minimum titer (1/1000) has high background. does any body has any suggestion for an anti his antibody with high sensitivity and specifity in crude cell lysate?

I am not sure why you chose to use an anti-Fab secondary antibody. What source is the recombinant protein that you're trying to express? If you don't see anything with the anti-his from Roche, you might have problems with the expression level. Be sure to run a WB with a positive control, so you'll at least know if the anti-his antibodies work. Are the anti-his monoclonal or polyclonal?