This assay has high sensitivity and excellent specificity for detection of Pig SERPINE1. No significant cross-reactivity or interference between Pig SERPINE1 and analogues was observed.

Samples

Serum, plasma, tissue homogenates

Assay Type

Sandwich

Detection Range

0.312 ng/ml-20 ng/ml

Sensitivity

0.078 ng/ml

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%Three samples of known concentration were tested twenty times on one plate to assess.Inter-assay Precision (Precision between assays): CV% is less than 10%Three samples of known concentration were tested in twenty assays to assess.

Detection Wavelength

450 nm

Sample Volume

50-100ul

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Product Note

Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Other Notes

Small volumes of SERPINE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PAI-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAI-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAI-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAI-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Function: Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.