GENOME ANNOUNCEMENT {#h0.0}
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*Escherichia coli* is a Gram-negative bacillus that is a component of the normal bacterial flora of the human colonic tract ([@B1][@B2][@B3]). *E. coli* within the intestinal tract is typically nonpathogenic unless the intestinal tract barriers have been violated or the host has been immunocompromised; however, there are also several highly adapted *E. coli* clones that have evolved to cause human diseases including meningitis, diarrhea, sepsis, and urinary tract infections ([@B3]). In fact, *E. coli* is the most common Gram-negative pathogen associated with nosocomial infections, and isolates with the ability to produce extended-spectrum β-lactamase (ESBL) continue to increase in frequency and severity ([@B4], [@B5]). The genomes of six ESBL-producing isolates obtained from wounded soldiers at the Walter Reed Army Medical Center (WRAMC) were sequenced using next-generation sequencing methods for future analyses to elucidate their virulence mechanisms.

As described previously, strains were routinely stored at −80°C in 10% glycerol ([@B6]). DNA was isolated from overnight LB cultures grown with agitation at 37°C using the DNeasy blood and tissue kit (Qiagen, Valencia, CA, USA). Absorption at 260 nm and 280 nm was measured for each sample to determine DNA quantity and quality using a Nanodrop 2000 (Thermo Scientific, Wilmington, DE, USA). DNA concentrations for library preparation were determined by the SYBR green (Life Technologies, Grand Island, NY, USA) standard curve method in black 96-well plates (Corning, Tewksbury, MA, USA) using a FilterMax F5 spectrophotometer with Multi-Mode Analysis software version 3.4.0.25 (Molecular Devices, Sunnyvale, CA, USA). Whole DNA was sheared to approximately 500 bp in a microTUBE-50 using the M220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). Fragmentation of resultant libraries was examined with a Bioanalyzer 2100 high sensitivity DNA analysis kit (Agilent Technologies, Santa Clara, CA, USA) using version B.02.08.SI648 software. Individual libraries were normalized, pooled, and then sequenced using MiSeq v3 600-cycle kit (Illumina, San Diego, CA, USA) to perform 300-bp paired-end sequencing on a MiSeq instrument (Illumina) per manufacturer's instructions. *De novo* assembly was performed using Genomics Workbench 8.0 with the bacterial genome finishing module (CLC bio, Boston, MA, USA) on a workstation with an AMD Opteron 2.10 GHz 16-core processor with 128 GB DDR3 ECC RAM. Prokka version 1.10 on a quadcore i7 workstation with 32 GB DDR3 running Ubuntu 14.04 LTS ([@B7]) was used for genome annotation. The *de novo* assembly statistics for the sequenced *E. coli* isolates are shown in [Table 1](#tab1){ref-type="table"}.

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Assembly metrics and accession numbers of *Escherichia coli* genomes

  Strain ID   No. of contigs   *N*~50~ contigs (bp)   Total size (bp)   Coverage (×)   G+C content (%)   No. of ORFs[^a^](#ngtab1.1){ref-type="table-fn"}   No. of RNAs   Accession no.
  ----------- ---------------- ---------------------- ----------------- -------------- ----------------- -------------------------------------------------- ------------- ------------------------------------------------------------------
  105454      101              191,011                5,335,253         16             50.68             5,121                                              70            [LOJL00000000](http://www.ncbi.nlm.nih.gov/nuccore/LOJL00000000)
  105547      93               127,705                4,858,207         18             50.68             4,649                                              74            [LOJM00000000](http://www.ncbi.nlm.nih.gov/nuccore/LOJM00000000)
  109497      99               222,697                5,221,557         34             50.78             5,050                                              74            [LORD00000000](http://www.ncbi.nlm.nih.gov/nuccore/LORD00000000)
  108191      98               115,521                4,727,016         28             50.81             4,518                                              73            [LORE00000000](http://www.ncbi.nlm.nih.gov/nuccore/LORE00000000)
  105433      89               138,092                4,820,700         33             50.74             4,556                                              73            [LORF00000000](http://www.ncbi.nlm.nih.gov/nuccore/LORF00000000)
  105438      81               204,373                5,226,940         34             50.68             4,996                                              60            [LORC00000000](http://www.ncbi.nlm.nih.gov/nuccore/LORC00000000)

Open reading frames.

Accession number(s). {#s1}
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The whole-genome shotgun projects were deposited into GenBank under Bioproject ID PRJNA261239 with the accession numbers listed in [Table 1](#tab1){ref-type="table"}.

**Citation** Arivett BA, Ream DC, Fiester SE, Kidane D, Actis LA. 2016. Draft genome sequences of *Escherichia coli* isolates from wounded military personnel. Genome Announc 4(4):e00828-16. doi:10.1128/genomeA.00828-16.

This work was supported by funds from Miami University and the United States Department of Defense grant W81XWH-12-2-0035 awarded to L.A.A.

We are grateful to Daniel V. Zurawski from Walter Reed Army Institute of Research for providing the *E. coli* strains listed in [Table 1](#tab1){ref-type="table"}. We would also like to thank Andor Kiss and the Miami University Center for Bioinformatics and Functional Genomics for assistance in sequence acquisition.

The findings and opinions expressed herein belong to the authors and do not necessarily reflect the official views of the WRAIR, the U.S. Army, or Department of Defense.
