An unexpected antiviral contribution of Mdm2 revealed by silencing/transit expression experiments. (A) The knock-down of p53 and/or Mdm2 mRNA expression in A549 cells differentially modulates levels of IAV production (left panel). Forty-eight hours after si-RNA transfection using a control si-RNAs (si-Ctrl) or specific siRNAs (
An unexpected antiviral contribution of Mdm2 revealed by silencing/transient expression experiments. ( A ) Knock-down of p53 and/or Mdm2 mRNA expression in A549 cells differentially modulates levels of IAV production (Left panel). Forty-eight hours after si-RNA transfection using a control si-RNAs (si-Ctrl) or specific siRNAs (si-p53/si-Mdm2), A549 cells were infected by A/Moscow/10/99 (H3N2) at a MOI of 0.01 and the level of viral production at 24 h post-infection was assessed using three different experiments: (i) RT-qPCR (log10 RNA copies/mL, measured in three independent experiments. An asterisk indicates a significant difference compared with the results for si-Ctlr treated cells ( *** and **** for respectively P -values 