Figure 6. Analysis of angiogenesis, apoptosis, and proliferation in MMTV-neu and Hoxb7/neu tumors. The arrows indicate representative blood vessels in both MMTV-neu and Hoxb7/neu tumors. The immunochemical staining analysis was performed as described in Materials and Methods. A, angiogenesis was assessed by counting blood vessels after immunosuppression with an
Figure 6. Analysis of angiogenesis, apoptosis, and proliferation in MMTV-neu and Hoxb7/neu tumors. The immunochemical staining analysis was performed as described in Materials and Methods. A, angiogenesis was assessed by counting blood vessels after immunostaining with an anti-CD34 antibody. A total of 33 pairs of tumors were analyzed. The average blood vessel index was presented by counting the number of blood vessels at 200× magnification. The arrows indicate representative blood vessels in both MMTV-neu and Hoxb7/neu tumors. B, apoptotic rate was analyzed by immunostaining analysis with antibody against active caspase-3. The arrows indicate positively staining cells. One thousand cells per tumor were counted at 400× magnification, and the average percentage of positive cells was presented. C, proliferation rate was assessed by immunochemical staining analysis. Fifteen pairs of randomly selected HER-2/neu and Hoxb7/HER-2/neu transgenic tumors were immunohistochemically examined using the anti-Ki67 antibody. One thousand tumor cells per tumor were counted, and the percentage of Ki67-positive cells was calculated. Arrows indicate some of the positive cells.