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<title>Plataforma LAMP para Diagnóstico Molecular de T. gondii e Giardia spp.</title>
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<h1 class="text-4xl md:text-5xl font-bold text-gray-800 leading-tight">
Plataforma LAMP para Diagnóstico Rápido de <span class="text-blue-600">T. gondii</span> e <span class="text-green-600">Giardia spp.</span>
</h1>
<p class="text-lg text-gray-600 mt-4">
Desenvolvimento e validação de um sistema portátil baseado na técnica LAMP alinhado à abordagem One Health
</p>
<div class="mt-8">
<a href="#methodology" class="bg-blue-600 text-white px-6 py-3 rounded-lg font-medium hover:bg-blue-700 transition duration-300">
Ver Metodologia
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<!-- Introduction Section -->
<section id="introduction" class="py-16 bg-white">
<div class="container mx-auto px-6">
<h2 class="text-3xl font-bold text-center text-gray-800 mb-12">Introdução</h2>
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<div class="flex items-center mb-4">
<div class="bg-blue-100 p-3 rounded-full mr-4">
<i class="fas fa-biohazard text-blue-600 text-xl"></i>
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<h3 class="text-xl font-semibold text-blue-800">Toxoplasma gondii</h3>
</div>
<p class="text-gray-700">
Toxoplasma gondii é um protozoário parasita intracelular obrigatório que infecta aproximadamente um terço da população humana mundial (Dubey, 2010). A infecção pode causar toxoplasmose congênita em gestantes e doença grave em imunocomprometidos.
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<i class="fas fa-parasite text-green-600 text-xl"></i>
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<h3 class="text-xl font-semibold text-green-800">Giardia spp.</h3>
</div>
<p class="text-gray-700">
Giardia duodenalis é um protozoário flagelado que causa giardíase, uma das principais doenças diarreicas em humanos e animais (Ryan & Cacciò, 2013). A transmissão ocorre via água ou alimentos contaminados, com impacto significativo na saúde pública.
</p>
</div>
</div>
<div class="mt-8 bg-gray-50 p-8 rounded-xl">
<h3 class="text-xl font-semibold text-gray-800 mb-4">One Health e ODS</h3>
<p class="text-gray-700 mb-4">
A abordagem One Health reconhece a interconexão entre saúde humana, animal e ambiental. Este projeto se alinha diretamente com os Objetivos de Desenvolvimento Sustentável (ODS) 3 (Saúde e Bem-estar), 9 (Indústria, Inovação e Infraestrutura) e 17 (Parcerias para os Objetivos).
</p>
<div class="flex flex-wrap gap-4 mt-4">
<span class="bg-blue-100 text-blue-800 px-4 py-2 rounded-full text-sm font-medium">ODS 3</span>
<span class="bg-purple-100 text-purple-800 px-4 py-2 rounded-full text-sm font-medium">ODS 9</span>
<span class="bg-green-100 text-green-800 px-4 py-2 rounded-full text-sm font-medium">ODS 17</span>
</div>
</div>
</div>
</section>
<!-- Justification Section -->
<section class="py-16 bg-gray-100">
<div class="container mx-auto px-6">
<h2 class="text-3xl font-bold text-center text-gray-800 mb-12">Justificativa</h2>
<div class="grid md:grid-cols-3 gap-8">
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<div class="text-yellow-500 mb-4">
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<h3 class="text-xl font-semibold mb-3">Limitações Diagnósticas</h3>
<p class="text-gray-600">
Os métodos atuais (microscopia, ELISA) apresentam baixa sensibilidade. O qPCR, apesar de sensível, requer equipamentos caros e mão de obra especializada, inviabilizando seu uso em campo.
</p>
</div>
<div class="bg-white p-6 rounded-xl shadow-sm">
<div class="text-blue-500 mb-4">
<i class="fas fa-lightbulb text-3xl"></i>
</div>
<h3 class="text-xl font-semibold mb-3">Vantagens da LAMP</h3>
<p class="text-gray-600">
A técnica LAMP (Loop-Mediated Isothermal Amplification) permite amplificação isotérmica rápida (60 min) com alta sensibilidade e especificidade, dispensando termocicladores (Notomi et al., 2000).
</p>
</div>
<div class="bg-white p-6 rounded-xl shadow-sm">
<div class="text-green-500 mb-4">
<i class="fas fa-mobile-alt text-3xl"></i>
</div>
<h3 class="text-xl font-semibold mb-3">Portabilidade</h3>
<p class="text-gray-600">
A adaptação da LAMP para um dispositivo portátil permitirá diagnóstico em tempo real em situações de campo, unidades básicas de saúde e vigilância ambiental, democratizando o acesso a diagnóstico molecular.
</p>
</div>
</div>
</div>
</section>
<!-- Objectives Section -->
<section class="py-16 bg-white">
<div class="container mx-auto px-6">
<h2 class="text-3xl font-bold text-center text-gray-800 mb-12">Objetivos</h2>
<div class="grid md:grid-cols-2 gap-8">
<div>
<h3 class="text-xl font-semibold text-blue-700 mb-4 flex items-center">
<span class="bg-blue-100 p-2 rounded-full mr-3">
<i class="fas fa-bullseye text-blue-600"></i>
</span>
Objetivo Geral
</h3>
<p class="text-gray-700 pl-10">
Desenvolver e validar uma plataforma de diagnóstico molecular portátil baseada na técnica LAMP para detecção simultânea de T. gondii e Giardia spp., alinhada aos princípios One Health.
</p>
</div>
<div>
<h3 class="text-xl font-semibold text-green-700 mb-4 flex items-center">
<span class="bg-green-100 p-2 rounded-full mr-3">
<i class="fas fa-list-ol text-green-600"></i>
</span>
Objetivos Específicos
</h3>
<ul class="text-gray-700 pl-10 space-y-2">
<li class="flex items-start">
<span class="text-green-500 mr-2">•</span> Selecionar e otimizar primers para os alvos REP-529 (T. gondii) e GDH (Giardia spp.)
</li>
<li class="flex items-start">
<span class="text-green-500 mr-2">•</span> Desenvolver protocolos LAMP multiplex
</li>
<li class="flex items-start">
<span class="text-green-500 mr-2">•</span> Validar frente ao qPCR padrão-ouro
</li>
<li class="flex items-start">
<span class="text-green-500 mr-2">•</span> Adaptar para dispositivo portátil com detecção colorimétrica
</li>
</ul>
</div>
</div>
</div>
</section>
<!-- Methodology Section -->
<section id="methodology" class="py-16 bg-gray-50">
<div class="container mx-auto px-6">
<h2 class="text-3xl font-bold text-center text-gray-800 mb-12">Metodologia</h2>
<!-- Primer Design -->
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<h3 class="text-2xl font-semibold text-gray-800 mb-6 flex items-center">
<span class="bg-purple-100 p-2 rounded-full mr-3">
<i class="fas fa-dna text-purple-600"></i>
</span>
Desenho de Primers In Silico
</h3>
<div class="grid md:grid-cols-2 gap-8">
<div>
<h4 class="text-lg font-medium text-purple-800 mb-3">Alvo REP-529 (T. gondii)</h4>
<p class="text-gray-700 mb-4">
O elemento repetitivo REP-529 (529 bp) foi selecionado por apresentar 200-300 cópias no genoma de T. gondii, garantindo alta sensibilidade (Homan et al., 2000).
</p>
<div class="bg-gray-100 p-4 rounded-lg overflow-x-auto">
<pre class="text-sm text-gray-800">
F3: 5'-GGA AGG CAA GTC TGG AGA AG-3'
B3: 5'-TGC ATC TGT AGG TCG AAC ATC-3'
FIP: 5'-TCC GCT GCA GAC ACA GTA TTC GGA GGG GAC GAA TTT CGT C-3'
BIP: 5'-AGC TTC CCT CGA ATG CAG TTC TTT TTG CGT TCA AAG CGT TCG-3'
LF: 5'-GCA GTA TCG TCG TGT GGT TC-3'
LB: 5'-CGC TTC TCG TTG TTG CTC TC-3'</pre>
</div>
</div>
<div>
<h4 class="text-lg font-medium text-green-800 mb-3">Alvo GDH (Giardia spp.)</h4>
<p class="text-gray-700 mb-4">
O gene da enzima glutamato desidrogenase (GDH) foi selecionado por sua conservação entre isolados de Giardia e diferenciação de assemblages (Amar et al., 2002).
</p>
<div class="bg-gray-100 p-4 rounded-lg overflow-x-auto">
<pre class="text-sm text-gray-800">
F3: 5'-GCA CGG ACG TTC CAG ACT-3'
B3: 5'-GCC TCG TCC ATC GCA A-3'
FIP: 5'-TCC TGG AGG AAG GAG CGA GAA GGT TTG GGT TCA TCG AAC C-3'
BIP: 5'-GGC TTC CGC TTC ATC GAC GTT TTT CAA CGT TCA CCG ACG AC-3'
LF: 5'-GAG CGA GGT GGA AAC ACC AA-3'
LB: 5'-GAC GAC CTC ACG GCA AAG TT-3'</pre>
</div>
</div>
</div>
<div class="mt-6 bg-blue-50 p-4 rounded-lg">
<p class="text-blue-800 flex items-start">
<span class="bg-blue-100 p-1 rounded-full mr-2">
<i class="fas fa-info-circle text-blue-600 text-sm"></i>
</span>
Os primers foram desenhados utilizando o software PrimerExplorer V5 (Eiken Chemical) e avaliados quanto a formação de estruturas secundárias (ΔG < -5 kcal/mol) e especificidade por BLASTn contra o banco de dados NCBI.
</p>
</div>
</div>
<!-- Workflow -->
<div class="mb-12">
<h3 class="text-2xl font-semibold text-gray-800 mb-6 text-center">Fluxograma Metodológico</h3>
<div class="bg-white p-6 rounded-xl">
<div class="flex flex-col md:flex-row justify-between items-center space-y-4 md:space-y-0">
<div class="text-center">
<div class="bg-purple-100 w-16 h-16 rounded-full flex items-center justify-center mx-auto mb-2">
<i class="fas fa-laptop-code text-purple-600 text-2xl"></i>
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<p class="font-medium">Análise In Silico</p>
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<div class="bg-blue-100 w-16 h-16 rounded-full flex items-center justify-center mx-auto mb-2">
<i class="fas fa-vial text-blue-600 text-2xl"></i>
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<p class="font-medium">Otimização In Vitro</p>
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<div class="text-center">
<div class="bg-green-100 w-16 h-16 rounded-full flex items-center justify-center mx-auto mb-2">
<i class="fas fa-vials text-green-600 text-2xl"></i>
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<p class="font-medium">Amostras Clínicas</p>
</div>
<div class="text-center">
<div class="bg-yellow-100 w-16 h-16 rounded-full flex items-center justify-center mx-auto mb-2">
<i class="fas fa-microscope text-yellow-600 text-2xl"></i>
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<p class="font-medium">Validação</p>
</div>
<div class="text-center">
<div class="bg-red-100 w-16 h-16 rounded-full flex items-center justify-center mx-auto mb-2">
<i class="fas fa-tablet-alt text-red-600 text-2xl"></i>
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<p class="font-medium">Dispositivo Portátil</p>
</div>
</div>
</div>
</div>
<!-- Experimental Design -->
<div class="grid md:grid-cols-3 gap-6 mb-12">
<div class="protocol-card bg-white p-6 rounded-xl shadow-sm transition duration-300">
<h4 class="text-lg font-semibold text-blue-800 mb-3 flex items-center">
<span class="bg-blue-100 p-2 rounded-full mr-2">
<i class="fas fa-flask text-blue-600"></i>
</span>
Reação LAMP
</h4>
<ul class="text-gray-700 space-y-2">
<li class="flex items-start">
<span class="text-blue-500 mr-2">•</span> Volume total: 25 μL
</li>
<li class="flex items-start">
<span class="text-blue-500 mr-2">•</span> Temperatura: 65°C
</li>
<li class="flex items-start">
<span class="text-blue-500 mr-2">•</span> Tempo: 60 min
</li>
<li class="flex items-start">
<span class="text-blue-500 mr-2">•</span> Indicador: HNB (azul→violeta)
</li>
</ul>
</div>
<div class="protocol-card bg-white p-6 rounded-xl shadow-sm transition duration-300">
<h4 class="text-lg font-semibold text-green-800 mb-3 flex items-center">
<span class="bg-green-100 p-2 rounded-full mr-2">
<i class="fas fa-dna text-green-600"></i>
</span>
Extração de DNA
</h4>
<ul class="text-gray-700 space-y-2">
<li class="flex items-start">
<span class="text-green-500 mr-2">•</span> Kit comercial (QIAamp)
</li>
<li class="flex items-start">
<span class="text-green-500 mr-2">•</span> Protocolo rápido (10 min)
</li>
<li class="flex items-start">
<span class="text-green-500 mr-2">•</span> Controle: C. parvum
</li>
</ul>
</div>
<div class="protocol-card bg-white p-6 rounded-xl shadow-sm transition duration-300">
<h4 class="text-lg font-semibold text-purple-800 mb-3 flex items-center">
<span class="bg-purple-100 p-2 rounded-full mr-2">
<i class="fas fa-chart-line text-purple-600"></i>
</span>
Validação
</h4>
<ul class="text-gray-700 space-y-2">
<li class="flex items-start">
<span class="text-purple-500 mr-2">•</span> Sensibilidade: Limite de detecção
</li>
<li class="flex items-start">
<span class="text-purple-500 mr-2">•</span> Especificidade: 100 isolados
</li>
<li class="flex items-start">
<span class="text-purple-500 mr-2">•</span> Comparação: qPCR TaqMan
</li>
</ul>
</div>
</div>
<!-- Portable Device -->
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<h3 class="text-2xl font-semibold text-gray-800 mb-6 flex items-center">
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<i class="fas fa-tablet-alt text-red-600"></i>
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Dispositivo Portátil
</h3>
<div class="grid md:grid-cols-2 gap-8 items-center">
<div>
<h4 class="text-lg font-medium text-gray-800 mb-3">Especificações Técnicas</h4>
<ul class="text-gray-700 space-y-3">
<li class="flex items-start">
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<i class="fas fa-battery-three-quarters text-red-600 text-xs"></i>
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Bateria recarregável (8h autonomia)
</li>
<li class="flex items-start">
<span class="bg-red-100 p-1 rounded-full mr-2">
<i class="fas fa-temperature-high text-red-600 text-xs"></i>
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Bloco térmico preciso (±0.5°C)
</li>
<li class="flex items-start">
<span class="bg-red-100 p-1 rounded-full mr-2">
<i class="fas fa-camera text-red-600 text-xs"></i>
</span>
Detecção colorimétrica por smartphone
</li>
<li class="flex items-start">
<span class="bg-red-100 p-1 rounded-full mr-2">
<i class="fas fa-weight text-red-600 text-xs"></i>
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Peso: <300g, dimensões: 12x8x4cm
</li>
</ul>
</div>
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<div class="relative">
<img src="https://img.icons8.com/color/512/test-tube.png" alt="Dispositivo Portátil" class="w-64 h-64">
<div class="absolute -bottom-5 -right-5 bg-white p-3 rounded-full shadow-lg">
<i class="fas fa-mobile-alt text-3xl text-blue-500"></i>
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</div>
</div>
</div>
</div>
</div>
</section>
<!-- Expected Results -->
<section class="py-16 bg-white">
<div class="container mx-auto px-6">
<h2 class="text-3xl font-bold text-center text-gray-800 mb-12">Resultados Esperados</h2>
<div class="grid md:grid-cols-2 gap-8">
<div>
<h3 class="text-xl font-semibold text-gray-800 mb-4 flex items-center">
<span class="bg-blue-100 p-2 rounded-full mr-3">
<i class="fas fa-chart-bar text-blue-600"></i>
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Desempenho Técnico
</h3>
<div class="bg-gray-50 p-6 rounded-xl">
<div class="mb-4">
<h4 class="font-medium text-gray-700 mb-2">Sensibilidade</h4>
<div class="w-full bg-gray-200 rounded-full h-4">
<div class="bg-blue-600 h-4 rounded-full" style="width: 95%"></div>
</div>
<p class="text-sm text-gray-600 mt-1">>95% comparado ao qPCR</p>
</div>
<div class="mb-4">
<h4 class="font-medium text-gray-700 mb-2">Especificidade</h4>
<div class="w-full bg-gray-200 rounded-full h-4">
<div class="bg-green-600 h-4 rounded-full" style="width: 98%"></div>
</div>
<p class="text-sm text-gray-600 mt-1">>98% sem reação cruzada</p>
</div>
<div>
<h4 class="font-medium text-gray-700 mb-2">Limite de Detecção</h4>
<div class="w-full bg-gray-200 rounded-full h-4">
<div class="bg-purple-600 h-4 rounded-full" style="width: 90%"></div>
</div>
<p class="text-sm text-gray-600 mt-1">1-10 cópias/μL</p>
</div>
</div>
</div>
<div>
<h3 class="text-xl font-semibold text-gray-800 mb-4 flex items-center">
<span class="bg-green-100 p-2 rounded-full mr-3">
<i class="fas fa-globe-americas text-green-600"></i>
</span>
Impacto Esperado
</h3>
<div class="space-y-4">
<div class="flex items-start bg-green-50 p-4 rounded-lg">
<div class="bg-green-100 p-2 rounded-full mr-3">
<i class="fas fa-clinic-medical text-green-600"></i>
</div>
<p class="text-gray-700">
Redução de custos diagnósticos em até 70% comparado a qPCR, permitindo implementação em unidades básicas de saúde
</p>
</div>
<div class="flex items-start bg-blue-50 p-4 rounded-lg">
<div class="bg-blue-100 p-2 rounded-full mr-3">
<i class="fas fa-microchip text-blue-600"></i>
</div>
<p class="text-gray-700">
Capacitação de profissionais para diagnóstico molecular em regiões remotas
</p>
</div>
</div>
</div>
</div>
</div>
</section>
<!-- Arduino Implementation -->
<section class="py-16 bg-gray-100">
<div class="container mx-auto px-6">
<h2 class="text-3xl font-bold text-center text-gray-800 mb-12">Implementação Arduino</h2>
<div class="grid md:grid-cols-2 gap-8">
<div>
<h3 class="text-xl font-semibold text-gray-800 mb-4 flex items-center">
<span class="bg-red-100 p-2 rounded-full mr-3">
<i class="fas fa-microchip text-red-600"></i>
</span>
Circuito do Dispositivo LAMP
</h3>
<div class="bg-white p-6 rounded-xl shadow-sm">
<h4 class="font-medium text-gray-700 mb-3">Componentes Principais:</h4>
<ul class="text-gray-700 space-y-2">
<li class="flex items-start">
<span class="text-red-500 mr-2">•</span> Arduino Nano (controle principal)
</li>
<li class="flex items-start">
<span class="text-red-500 mr-2">•</span> Módulo Peltier (controle térmico)
</li>
<li class="flex items-start">
<span class="text-red-500 mr-2">•</span> Sensor DS18B20 (monitoramento de temperatura)
</li>
<li class="flex items-start">
<span class="text-red-500 mr-2">•</span> Módulo Bluetooth HC-05 (conexão com smartphone)
</li>
<li class="flex items-start">
<span class="text-red-500 mr-2">•</span> LED RGB (indicação visual dos resultados)
</li>
</ul>
</div>
</div>
<div>
<h3 class="text-xl font-semibold text-gray-800 mb-4 flex items-center">
<span class="bg-blue-100 p-2 rounded-full mr-3">
<i class="fas fa-code text-blue-600"></i>
</span>
Código Principal
</h3>
<div class="bg-gray-800 p-4 rounded-xl overflow-x-auto">
<pre class="text-sm text-gray-300">
#include <OneWire.h>
#include <DallasTemperature.h>
#define ONE_WIRE_BUS 2
OneWire oneWire(ONE_WIRE_BUS);
DallasTemperature sensors(&oneWire);
const int peltierPin = 3;
const int ledPin = 4;
float targetTemp = 65.0;
void setup() {
Serial.begin(9600);
sensors.begin();
pinMode(peltierPin, OUTPUT);
pinMode(ledPin, OUTPUT);
}
void loop() {
sensors.requestTemperatures();
float currentTemp = sensors.getTempCByIndex(0);
// Controle PID simplificado
if(currentTemp < targetTemp - 0.5) {
digitalWrite(peltierPin, HIGH);
} else if(currentTemp > targetTemp + 0.5) {
digitalWrite(peltierPin, LOW);
}
// Monitoramento serial
Serial.print("Temperature: ");
Serial.print(currentTemp);
Serial.println(" °C");
delay(1000);
}</pre>
</div>
</div>
</div>
<div class="mt-8 bg-white p-6 rounded-xl shadow-sm">
<h3 class="text-xl font-semibold text-gray-800 mb-4 flex items-center">
<span class="bg-green-100 p-2 rounded-full mr-3">
<i class="fas fa-project-diagram text-green-600"></i>
</span>
Fluxo de Operação
</h3>
<div class="grid md:grid-cols-4 gap-4 text-center">
<div class="bg-green-50 p-4 rounded-lg">
<div class="bg-green-100 w-12 h-12 rounded-full flex items-center justify-center mx-auto mb-2">
<i class="fas fa-power-off text-green-600"></i>
</div>
<p class="font-medium">1. Ligar dispositivo</p>
</div>
<div class="bg-blue-50 p-4 rounded-lg">
<div class="bg-blue-100 w-12 h-12 rounded-full flex items-center justify-center mx-auto mb-2">
<i class="fas fa-temperature-high text-blue-600"></i>
</div>
<p class="font-medium">2. Aquecer a 65°C</p>
</div>
<div class="bg-purple-50 p-4 rounded-lg">
<div class="bg-purple-100 w-12 h-12 rounded-full flex items-center justify-center mx-auto mb-2">
<i class="fas fa-vial text-purple-600"></i>
</div>
<p class="font-medium">3. Inserir amostra</p>
</div>
<div class="bg-red-50 p-4 rounded-lg">
<div class="bg-red-100 w-12 h-12 rounded-full flex items-center justify-center mx-auto mb-2">
<i class="fas fa-mobile-alt text-red-600"></i>
</div>
<p class="font-medium">4. Ler resultados</p>
</div>
</div>
</div>
<!-- Industrial Design and Assembly -->
<div class="mt-12">
<h3 class="text-2xl font-bold text-center text-gray-800 mb-8">Desenho Industrial e Montagem</h3>
<div class="bg-white p-8 rounded-xl shadow-lg">
<div class="grid md:grid-cols-2 gap-8 items-center mb-8">
<div>
<h4 class="text-xl font-semibold text-gray-800 mb-4">Especificações do Design</h4>
<ul class="text-gray-700 space-y-3">
<li class="flex items-start">
<span class="bg-blue-100 p-1 rounded-full mr-2">
<i class="fas fa-ruler-combined text-blue-600 text-xs"></i>
</span>
Dimensões: 120mm x 80mm x 40mm
</li>
<li class="flex items-start">
<span class="bg-blue-100 p-1 rounded-full mr-2">
<i class="fas fa-box-open text-blue-600 text-xs"></i>
</span>
Carcaça em ABS impressa em 3D
</li>
<li class="flex items-start">
<span class="bg-blue-100 p-1 rounded-full mr-2">
<i class="fas fa-thermometer-half text-blue-600 text-xs"></i>
</span>
Compartimento térmico isolado
</li>
<li class="flex items-start">
<span class="bg-blue-100 p-1 rounded-full mr-2">
<i class="fas fa-eye text-blue-600 text-xs"></i>
</span>
Janela transparente para observação
</li>
</ul>
</div>
<div class="flex justify-center">
<img src="https://img.icons8.com/color/512/3d-print.png" alt="Design 3D" class="w-64 h-64">
</div>
</div>
<h4 class="text-xl font-semibold text-gray-800 mb-4">Diagrama de Montagem</h4>
<div class="bg-gray-100 p-4 rounded-lg mb-8">
<img src="https://img.icons8.com/color/512/electrical.png" alt="Diagrama de Circuito" class="w-full max-w-2xl mx-auto">
</div>
<h4 class="text-xl font-semibold text-gray-800 mb-4">Passo a Passo de Montagem</h4>
<div class="space-y-4">
<div class="flex items-start bg-blue-50 p-4 rounded-lg">
<div class="bg-blue-100 p-2 rounded-full mr-4">
<span class="font-bold text-blue-800">1</span>
</div>
<div>
<h5 class="font-medium text-blue-800">Preparação da Carcaça</h5>
<p class="text-gray-700">Imprima as peças em 3D usando ABS. Lixe as rebarbas e encaixe as partes para verificar o ajuste.</p>
</div>
</div>
<div class="flex items-start bg-green-50 p-4 rounded-lg">
<div class="bg-green-100 p-2 rounded-full mr-4">
<span class="font-bold text-green-800">2</span>
</div>
<div>
<h5 class="font-medium text-green-800">Instalação do Sistema Térmico</h5>
<p class="text-gray-700">Cole o módulo Peltier no bloco de alumínio usando pasta térmica. Instale o sensor DS18B20 próximo ao centro.</p>
</div>
</div>
<div class="flex items-start bg-purple-50 p-4 rounded-lg">
<div class="bg-purple-100 p-2 rounded-full mr-4">
<span class="font-bold text-purple-800">3</span>
</div>
<div>
<h5 class="font-medium text-purple-800">Montagem da Placa</h5>
<p class="text-gray-700">Soldar todos os componentes na protoboard conforme diagrama. Conectar o Arduino Nano, módulo Bluetooth e LED RGB.</p>
</div>
</div>
<div class="flex items-start bg-yellow-50 p-4 rounded-lg">
<div class="bg-yellow-100 p-2 rounded-full mr-4">
<span class="font-bold text-yellow-800">4</span>
</div>
<div>
<h5 class="font-medium text-yellow-800">Integração Final</h5>
<p class="text-gray-700">Fixar a placa na carcaça inferior. Conectar os fios do Peltier e sensor. Ajustar a janela transparente.</p>
</div>
</div>
<div class="flex items-start bg-red-50 p-4 rounded-lg">
<div class="bg-red-100 p-2 rounded-full mr-4">
<span class="font-bold text-red-800">5</span>
</div>
<div>
<h5 class="font-medium text-red-800">Testes Finais</h5>
<p class="text-gray-700">Ligar o dispositivo e verificar: controle de temperatura, comunicação Bluetooth e indicação LED.</p>
</div>
</div>
</div>
</div>
</div>
</div>
</section>
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