<p>I managed to figure out the answer to my question. I installed Bio::Tools::Primer3 by typing the following into terminal: '<code>sudo cpanm Bio::Tools::Primer3 --force</code>' to install it. It only works when I used 'sudo' and 'force'</p>

CC BY-SA 4.0
2020-12-27T19:46:03.503Z
2020-12-27T19:46:03.503Z
ShawnC
<ol>
<li>Hi there I am still a novice at this. I'm trying to run this perl script called FullSSR <a href="https://sourceforge.net/projects/fullssr" rel="nofollow noreferrer">https://sourceforge.net/projects/fullssr</a>, by following the instructions in their 'README.TXT' <a href="https://sourceforge.net/projects/fullssr/files/" rel="nofollow noreferrer">https://sourceforge.net/projects/fullssr/files/</a>.</li>
<li>I did everything as instructed by the app developer(s) which included among others, replacing the Primer3.pm file provided in the *.tar.gz file into /usr/share/perl5/Bio/Tools/Run/; opening the config.txt and specifying the path of primer3_core (file) and primer3_config/ (folder); placing the sequence file I wanted to analyse into the FullSSR folder and executing the perl script using the command line: <code>perl 'FullSSR\(ver-1.1\).p; -i sequence.fasta -m 5</code>.</li>
<li>However, it was to no avail. Instead, I got the message &quot;Can't locate object method &quot;new&quot; via package Bio::Tools::Primer3&quot; (perhaps you forgot to load &quot;Bio::Tools::Primer3?) at /usr/share/perl5/Bio/Tools/Run/Primer3.pm line 497.</li>
<li>I am going to try to install/load Bio::Tools::Primer3 with the cpan module (cpanm) and will see if that does it.</li>
<li>Perhaps someone out there could suggest a helpful (easy to understand) manual on CPAN?!</li>
</ol>

CC BY-SA 4.0
2020-12-27T19:41:46.757Z
I managed to figure out the answer to my question. I installed Bio::Tools::Primer3 by typing the following into terminal: 'sudo cpanm Bio::Tools::Primer3 --force' to install it. It only works when I used 'sudo' and 'force'
ShawnC
<perl>
Can't locate object method "new" via package Bio::Tools::Primer3
# Can't locate object method "new" via package Bio::Tools::Primer3

(Asked by: username_0 on 2020-12-27)

1. Hi there I am still a novice at this. I'm trying to run this perl script called FullSSR <https://sourceforge.net/projects/fullssr>, by following the instructions in their 'README.TXT' <https://sourceforge.net/projects/fullssr/files/>.
2. I did everything as instructed by the app developer(s) which included among others, replacing the Primer3.pm file provided in the \*.tar.gz file into /usr/share/perl5/Bio/Tools/Run/; opening the config.txt and specifying the path of primer3\_core (file) and primer3\_config/ (folder); placing the sequence file I wanted to analyse into the FullSSR folder and executing the perl script using the command line: `perl 'FullSSR\(ver-1.1\).p; -i sequence.fasta -m 5`.
3. However, it was to no avail. Instead, I got the message "Can't locate object method "new" via package Bio::Tools::Primer3" (perhaps you forgot to load "Bio::Tools::Primer3?) at /usr/share/perl5/Bio/Tools/Run/Primer3.pm line 497.
4. I am going to try to install/load Bio::Tools::Primer3 with the cpan module (cpanm) and will see if that does it.
5. Perhaps someone out there could suggest a helpful (easy to understand) manual on CPAN?!

**Tags:** perl
> **Comments:**
> **username_0**: I managed to figure out the answer to my question. I installed Bio::Tools::Primer3 by typing the following into terminal: 'sudo cpanm Bio::Tools::Primer3 --force' to install it. It only works when I used 'sudo' and 'force'  

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## 1 answers

### Answer by username_0 on 2020-12-27. Score: 1

I managed to figure out the answer to my question. I installed Bio::Tools::Primer3 by typing the following into terminal: '`sudo cpanm Bio::Tools::Primer3 --force`' to install it. It only works when I used 'sudo' and 'force'


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