<p>slingshot (Bioconductor) accepts a priori starts and ends. For differential expression along the trajectory you may check tradeSeq.</p>

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2023-06-19T21:20:11.630Z
2023-06-19T21:20:11.630Z
ATpoint
<p>I have single cell data from two samples : normal and pathological and
I would like to track the progression of the cells (and find the genes that drive it) from normal to pathological.</p>
<p>Upon first glance, it seems most reasonable to define the trajectory <strong>in advance</strong> in such a way that it would have a starting point, an end point, and a straight route between these two.
Is it possible, and using what package if so?</p>
<p>P.S. In contrast to the question above - I started with the <em>destiny</em> Bioconductor package. However, it always yields three tips for the diffusion pseudotime, and I am not sure how to determine the starting and the end tip of the trajectory...</p>

<bioconductor><single-cell>
What trajectory analysis method allows to set the form of the trajectory?
# What trajectory analysis method allows to set the form of the trajectory?

(Asked by: username_0 on 2023-06-19)

I have single cell data from two samples : normal and pathological and
I would like to track the progression of the cells (and find the genes that drive it) from normal to pathological.

Upon first glance, it seems most reasonable to define the trajectory **in advance** in such a way that it would have a starting point, an end point, and a straight route between these two.
Is it possible, and using what package if so?

P.S. In contrast to the question above - I started with the *destiny* Bioconductor package. However, it always yields three tips for the diffusion pseudotime, and I am not sure how to determine the starting and the end tip of the trajectory...

**Tags:** bioconductor, single-cell

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## 1 answers

### Answer by username_1 on 2023-06-19. Score: 4 | Accepted answer

slingshot (Bioconductor) accepts a priori starts and ends. For differential expression along the trajectory you may check tradeSeq.


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