<p>Yes, Prokka will consider all six-frames, the gene prediction is actually done with Prodigal, paper:
<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848648/" rel="noreferrer">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848648/</a></p>

<p>github:
<a href="https://github.com/hyattpd/Prodigal" rel="noreferrer">https://github.com/hyattpd/Prodigal</a></p>

CC BY-SA 4.0
2018-12-27T21:42:52.467Z
2018-12-27T21:42:52.467Z
Chris_Rands
<p>Does Prokka do six-frame genome annotations? The corresponding publication does not mention it.</p>

<blockquote>
  <p>Seemann, Torsten. 2014. “Prokka: Rapid Prokaryotic Genome Annotation.” Bioinformatics  30 (14): 2068–69.</p>
</blockquote>

<sequence-annotation>
Does Prokka do six-frame translations?
# Does Prokka do six-frame translations?

(Asked by: username_0 on 2018-12-27)

Does Prokka do six-frame genome annotations? The corresponding publication does not mention it.

> Seemann, Torsten. 2014. “Prokka: Rapid Prokaryotic Genome Annotation.” Bioinformatics 30 (14): 2068–69.

**Tags:** sequence-annotation

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## 1 answers

### Answer by username_1 on 2018-12-27. Score: 7 | Accepted answer

Yes, Prokka will consider all six-frames, the gene prediction is actually done with Prodigal, paper:
<https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848648/>

github:
<https://github.com/hyattpd/Prodigal>


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