<p>There's nothing really special about RNA alignments, you can use any alignment editor, including whichever one you use for protein. That said, a classic and very useful tool for this sort of thing is <a href="http://www.jalview.org/" rel="noreferrer">JalView</a>. It can be installed locally or run as a Java webapp from your browser.</p>

<blockquote>
  <p>Jalview has built in DNA, RNA and protein sequence and structure visualisation and analysis capabilities. It uses Jmol to view 3D structures, and VARNA to display RNA secondary structure. The Jalview Desktop can also connect with databases and analysis services, and provides a graphical interface to the alignment and analysis services provided by the JavA Bioinformatics Analysis Web Services framework.</p>
</blockquote>

<p><a href="https://i.sstatic.net/I76Yq.png" rel="noreferrer"><img src="https://i.sstatic.net/I76Yq.png" alt="jalview image"></a></p>

CC BY-SA 3.0
2017-06-08T09:22:17.537Z
2017-06-08T09:22:17.537Z
terdon
<p>I would suggest use RALEE—RNALignment Editor in Emacs. It can get for you the consensus secondary structure, you can move left/right sequences and their secondary structures (you can't do it in JalView!), and more.</p>

<p>It's an Emacs mode, so could be a bit hard to start off, but just try, you don't have to use all Emacs features to edit your alignments! </p>

<blockquote>
  <p>The RALEE (RNA ALignment Editor in Emacs) tool provides a simple
  environment for RNA multiple sequence alignment editing, including
  structure-specific colour schemes, utilizing helper applications for
  structure prediction and many more conventional editing functions.</p>
</blockquote>

<p><a href="https://doi.org/10.1093/bioinformatics/bth489" rel="nofollow noreferrer">Sam Griffiths-Jones Bioinformatics (2005) 21 (2): 257-259.</a></p>

<p><a href="https://i.sstatic.net/XOWVV.png" rel="nofollow noreferrer"><img src="https://i.sstatic.net/XOWVV.png" alt="enter image description here"></a></p>

<p><a href="https://i.sstatic.net/UPcwI.png" rel="nofollow noreferrer"><img src="https://i.sstatic.net/UPcwI.png" alt="enter image description here"></a>
Fig. You can move left/right sequences and their secondary structures (you can't do it in JalView!)</p>

CC BY-SA 3.0
2017-06-08T09:40:49.397Z
Ha! +1 for pure geekiness and for using the world's best editor! :P Are you sure about jalview not supporting such moving though? I haven't used it in a while but as I recall you could just select multiple sequences (and the structure line is just another "sequence") and any edit applied to one would be applied to all. Is that wrong?
terdon
CC BY-SA 3.0
2017-06-08T15:14:19.393Z
Since I use emacs, I think this will be really good for me ! Thanks.
Peter
CC BY-SA 4.0
2017-06-08T09:23:18.300Z
2018-12-31T05:43:06.480Z
2018-12-31T05:43:06.480Z
Marcin Magnus
<p>I have many alignments from Rfam Database, and I would like to edit them. 
I saw that many tools are used for Protein sequence alignments, but there is something specific to edit RNA alignments ? </p>

<p>e.g. Stockholm Alignment of Pistol (just few entries). </p>

<pre><code>FP929053.1/1669026-1668956   AGUGGUCACAGCCACUAUAAACA-GGGCUU-UAAGCUGUG-AGCGUUGACCGUC----------ACAA-----CGGCGGUCAGGUAGUC
AFOX01000025.1/1981-1912     ACUCGUCUGAGCGAGUAUAAACA-GGUCAU-UAAGCUCAG-AGCGUUCACCGGG----------AUCA------UUCGGUGAGGUUGGC
HE577054.1/3246821-3246752   ACUCGUCUGAGCGAGUAUAAACA-GGUCAU-UAAGCUCAG-AGCGUUCACCGGG----------AUCA------UGCGGUGAGGUUGGC
CP000154.1/3364237-3364168   GUUCGUCUGAGCGAACGCAAACA-GGCCAU-UAAGCUCAG-AGCGUUCACUGGA----------UUCG------UCCAGUGAGAUUGGC`
`#=GC SS_cons                 &lt;&lt;&lt;&lt;__AAAAA_&gt;&gt;&gt;&gt;-------..&lt;&lt;&lt;&lt;-.----aaaaa.----&lt;&lt;&lt;&lt;&lt;&lt;&lt;&lt;&lt;..........____....._&gt;&gt;&gt;&gt;&gt;&gt;&gt;&gt;&gt;--&gt;&gt;&gt;&gt;`
`#=GC RF                      acUCGUCuggGCGAguAUAAAuA..cgCaU.UAgGCccaG.AGCGUcccggcgg..........uUau.....uccgccgggGGUuGcg
//
</code></pre>

CC BY-SA 3.0
2017-06-08T09:19:13.373Z
Is there any reason you can't use your preferred tool for editing protein alignments? The format is the same, I've never come across an alignment editor that can deal with protein but not nucleotide alignments.
terdon
CC BY-SA 3.0
2017-06-08T10:18:08.523Z
Yes, because I do not have a preferred tool yet, and this question would broad my horizon of possibilities. Since it is possible that someone that use to edit RNAs has a preference between software because, maybe, one is more optimized than another. Since the bioinformatic is so vast and new tools are born every day, I don't see how this could be a stupid question.
Peter
CC BY-SA 3.0
2017-06-08T11:06:32.350Z
Oh, hey, nobody said it was a stupid question! I was just asking why you specified RNA in case you needed a specific feature regarding it. In general, a sequence alignment is a sequence alignment and it doesn't make much difference if it is protein, DAN or RNA. That said, RALEE does have some RNA_specific features.
terdon
<rna><rna-alignment>
What to use to edit RNA alignments?
# What to use to edit RNA alignments?

(Asked by: username_0 on 2017-06-08)

I have many alignments from Rfam Database, and I would like to edit them. 
I saw that many tools are used for Protein sequence alignments, but there is something specific to edit RNA alignments ? 
e.g. Stockholm Alignment of Pistol (just few entries). 
FP929053.1/1669026-1668956   AGUGGUCACAGCCACUAUAAACA-GGGCUU-UAAGCUGUG-AGCGUUGACCGUC----------ACAA-----CGGCGGUCAGGUAGUC
AFOX01000025.1/1981-1912     ACUCGUCUGAGCGAGUAUAAACA-GGUCAU-UAAGCUCAG-AGCGUUCACCGGG----------AUCA------UUCGGUGAGGUUGGC
HE577054.1/3246821-3246752   ACUCGUCUGAGCGAGUAUAAACA-GGUCAU-UAAGCUCAG-AGCGUUCACCGGG----------AUCA------UGCGGUGAGGUUGGC
CP000154.1/3364237-3364168   GUUCGUCUGAGCGAACGCAAACA-GGCCAU-UAAGCUCAG-AGCGUUCACUGGA----------UUCG------UCCAGUGAGAUUGGC`
`#=GC SS_cons                 <<<<__AAAAA_>>>>-------..<<<<-.----aaaaa.----<<<<<<<<<..........____....._>>>>>>>>>-->>>>`
`#=GC RF                      acUCGUCuggGCGAguAUAAAuA..cgCaU.UAgGCccaG.AGCGUcccggcgg..........uUau.....uccgccgggGGUuGcg
//

**Tags:** rna, rna-alignment
> **Comments:**
> **username_1**: Is there any reason you can't use your preferred tool for editing protein alignments? The format is the same, I've never come across an alignment editor that can deal with protein but not nucleotide alignments.  
> **username_0**: Yes, because I do not have a preferred tool yet, and this question would broad my horizon of possibilities. Since it is possible that someone that use to edit RNAs has a preference between software because, maybe, one is more optimized than another. Since the bioinformatic is so vast and new tools are born every day, I don't see how this could be a stupid question.  
> **username_1**: Oh, hey, nobody said it was a stupid question! I was just asking why you specified RNA in case you needed a specific feature regarding it. In general, a sequence alignment is a sequence alignment and it doesn't make much difference if it is protein, DAN or RNA. That said, RALEE does have some RNA_specific features.  

---

## 2 answers

### Answer by username_2 on 2017-06-08. Score: 11 | Accepted answer

I would suggest use RALEE—RNALignment Editor in Emacs. It can get for you the consensus secondary structure, you can move left/right sequences and their secondary structures (you can't do it in JalView!), and more.

It's an Emacs mode, so could be a bit hard to start off, but just try, you don't have to use all Emacs features to edit your alignments!

> The RALEE (RNA ALignment Editor in Emacs) tool provides a simple
> environment for RNA multiple sequence alignment editing, including
> structure-specific colour schemes, utilizing helper applications for
> structure prediction and many more conventional editing functions.

[Sam Griffiths-Jones Bioinformatics (2005) 21 (2): 257-259.](https://doi.org/10.1093/bioinformatics/bth489)

[![enter image description here](https://i.sstatic.net/XOWVV.png)](https://i.sstatic.net/XOWVV.png)

[![enter image description here](https://i.sstatic.net/UPcwI.png)](https://i.sstatic.net/UPcwI.png)
Fig. You can move left/right sequences and their secondary structures (you can't do it in JalView!)

> **Comments:**
> **username_1**: Ha! +1 for pure geekiness and for using the world's best editor! :P Are you sure about jalview not supporting such moving though? I haven't used it in a while but as I recall you could just select multiple sequences (and the structure line is just another "sequence") and any edit applied to one would be applied to all. Is that wrong?  
> **username_0**: Since I use emacs, I think this will be really good for me ! Thanks.  

---

### Answer by username_1 on 2017-06-08. Score: 7

There's nothing really special about RNA alignments, you can use any alignment editor, including whichever one you use for protein. That said, a classic and very useful tool for this sort of thing is [JalView](http://www.jalview.org/). It can be installed locally or run as a Java webapp from your browser.

> Jalview has built in DNA, RNA and protein sequence and structure visualisation and analysis capabilities. It uses Jmol to view 3D structures, and VARNA to display RNA secondary structure. The Jalview Desktop can also connect with databases and analysis services, and provides a graphical interface to the alignment and analysis services provided by the JavA Bioinformatics Analysis Web Services framework.

[![jalview image](https://i.sstatic.net/I76Yq.png)](https://i.sstatic.net/I76Yq.png)


---

