<p><a href="https://bioconductor.org/packages/release/bioc/vignettes/QuasR/inst/doc/QuasR.html" rel="nofollow noreferrer">The QuasR vignette</a> tells you how to run it. The samples file should contain a list of your samples and fastq files.</p>

CC BY-SA 4.0
2022-05-14T16:53:20.457Z
2022-05-14T16:53:20.457Z
Cloudberry
<p>I'm an absolute beginner trying to solve this question &quot;Align the trimmed and untrimmed reads using QuasR and plot alignment statistics, did the trimming improve alignments?&quot;</p>
<p>I did trim the reads using the preprocessReads() function, now I want to align the output but the qAlign() only takes a samples file and genome file. however, my trimmed reads are two separate files how can I align them.</p>
<p>I asked google but I didn't find the answer</p>

CC BY-SA 4.0
2022-05-14T16:33:40.623Z
Please clarify your specific problem or provide additional details to highlight exactly what you need. As it's currently written, it's hard to tell exactly what you're asking.
Community
CC BY-SA 4.0
2023-09-15T20:41:39.533Z
Please consider the answer below and whether you wish to upvote/accept.
M__
<r><rna-seq><phylogenetics><homework>
how to create "sample file" for the qAlign() function after trimming the reads in R
# how to create "sample file" for the qAlign() function after trimming the reads in R

(Asked by: username_0 on 2022-05-14)

I'm an absolute beginner trying to solve this question "Align the trimmed and untrimmed reads using QuasR and plot alignment statistics, did the trimming improve alignments?"

I did trim the reads using the preprocessReads() function, now I want to align the output but the qAlign() only takes a samples file and genome file. however, my trimmed reads are two separate files how can I align them.

I asked google but I didn't find the answer

**Tags:** r, rna-seq, phylogenetics, homework
> **Comments:**
> **username_1**: Please clarify your specific problem or provide additional details to highlight exactly what you need. As it's currently written, it's hard to tell exactly what you're asking.  
> **username_2**: Please consider the answer below and whether you wish to upvote/accept.  

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## 1 answers

### Answer by username_3 on 2022-05-14. Score: 1

[The QuasR vignette](https://bioconductor.org/packages/release/bioc/vignettes/QuasR/inst/doc/QuasR.html) tells you how to run it. The samples file should contain a list of your samples and fastq files.


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