<p>Depending on how long the sequences are, you could use the LAST Web Service:</p>

<p><a href="http://lastweb.cbrc.jp/" rel="nofollow noreferrer">http://lastweb.cbrc.jp/</a></p>

<p>It has an HTML view that shows base-level differences with colour-coding.</p>

<p>LAST [download / command-line version] can also be used for producing whole-genome comparison dot-plots.</p>

<p>Another thing I do for comparing assemblies is to chop one assembly into smaller pieces (e.g. 10kb), map the pieces to other other assembly to generate BAM output, then look at the BAM pileup file in a BAM viewer (e.g. <a href="https://ics.hutton.ac.uk/tablet/download-tablet/" rel="nofollow noreferrer">Tablet</a>).</p>

CC BY-SA 4.0
2020-03-01T21:44:21.447Z
2020-03-01T21:44:21.447Z
gringer
<p>You can try <a href="http://darlinglab.org/mauve/mauve.html" rel="nofollow noreferrer">Mauve</a> multiple genome aligner.</p>

<p><a href="https://i.sstatic.net/tWfoR.png" rel="nofollow noreferrer"><img src="https://i.sstatic.net/tWfoR.png" alt="enter image description here"></a></p>

CC BY-SA 4.0
2020-03-01T00:09:46.280Z
Mauve is a very good tool for this! Thanks. Does Mauve allow for us to visualize base-level differences (easily)?
EB2127
CC BY-SA 4.0
2020-03-01T21:18:56.360Z
I am not sure. I have not tried it at that resolution and i have not used it in a long time.
burger
CC BY-SA 4.0
2020-02-29T23:16:13.777Z
2020-02-29T23:16:13.777Z
burger
<p>Before you compare your genome assembly fasta, I would recommend you first do a reference guided scaffolding using the tool <a href="https://github.com/malonge/RagTag" rel="nofollow noreferrer">RagTag</a>.</p>
<p>Once you are done you can compare the newly generated scaffolds (with other genomes or not) using the software BRIG. I made a video of how to use this software <a href="https://youtu.be/pobQgE4z-5Q" rel="nofollow noreferrer">here</a></p>
<p>In addition you can also do a genome annotation on your scaffold using prokka. I made a video for that as well <a href="https://youtu.be/MqDhhuIXjgg" rel="nofollow noreferrer">here</a></p>
<p>Good luck</p>

CC BY-SA 4.0
2021-01-02T22:48:36.153Z
2021-01-02T22:48:36.153Z
Kobina
<p>For whole-genome align:
Maybe you could use mummer? Mummer is good to align the whole genome of species, and it also has so many other tools to modify the result. (It can show the SNP.)</p>

<p>For short sequence:
You could try mega X</p>

CC BY-SA 4.0
2020-03-09T10:17:20.820Z
2020-03-09T10:17:20.820Z
TANGLi83
<p>I have two FASTAs/assemblies of the same species, but the bases are somewhat different. I would like to explore this. </p>

<p>What tools/methods exist to compare two FASTAs and see the difference in structure between them? Are there visualization tools available, something like IGV? </p>

<p>I think users would need to do a multiple-sequence alignment between these FASTAs, and then check for the differences between them. However, I don't know if a "standard approach" exists to do this. </p>

<phylogenetics><fasta><assembly><visualization>
Tools for comparing/visualizing FASTAs?
# Tools for comparing/visualizing FASTAs?

(Asked by: username_0 on 2020-02-29)

I have two FASTAs/assemblies of the same species, but the bases are somewhat different. I would like to explore this.

What tools/methods exist to compare two FASTAs and see the difference in structure between them? Are there visualization tools available, something like IGV?

I think users would need to do a multiple-sequence alignment between these FASTAs, and then check for the differences between them. However, I don't know if a "standard approach" exists to do this.

**Tags:** phylogenetics, fasta, assembly, visualization

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## 4 answers

### Answer by username_1 on 2020-03-01. Score: 2

Depending on how long the sequences are, you could use the LAST Web Service:

<http://lastweb.cbrc.jp/>

It has an HTML view that shows base-level differences with colour-coding.

LAST [download / command-line version] can also be used for producing whole-genome comparison dot-plots.

Another thing I do for comparing assemblies is to chop one assembly into smaller pieces (e.g. 10kb), map the pieces to other other assembly to generate BAM output, then look at the BAM pileup file in a BAM viewer (e.g. [Tablet](https://ics.hutton.ac.uk/tablet/download-tablet/)).


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### Answer by username_2 on 2020-02-29. Score: 2

You can try [Mauve](http://darlinglab.org/mauve/mauve.html) multiple genome aligner.

[![enter image description here](https://i.sstatic.net/tWfoR.png)](https://i.sstatic.net/tWfoR.png)

> **Comments:**
> **username_0**: Mauve is a very good tool for this! Thanks. Does Mauve allow for us to visualize base-level differences (easily)?  
> **username_2**: I am not sure. I have not tried it at that resolution and i have not used it in a long time.  

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### Answer by username_3 on 2021-01-02. Score: 1

Before you compare your genome assembly fasta, I would recommend you first do a reference guided scaffolding using the tool [RagTag](https://github.com/malonge/RagTag).

Once you are done you can compare the newly generated scaffolds (with other genomes or not) using the software BRIG. I made a video of how to use this software [here](https://youtu.be/pobQgE4z-5Q)

In addition you can also do a genome annotation on your scaffold using prokka. I made a video for that as well [here](https://youtu.be/MqDhhuIXjgg)

Good luck


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### Answer by username_4 on 2020-03-09. Score: 1

For whole-genome align:
Maybe you could use mummer? Mummer is good to align the whole genome of species, and it also has so many other tools to modify the result. (It can show the SNP.)

For short sequence:
You could try mega X


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