<p>Just go to Blast <a href="https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&amp;PAGE_TYPE=BlastSearch&amp;LINK_LOC=blasthome" rel="nofollow noreferrer">here</a></p>
<ol>
<li><p>Click where is says Database go to Transcriptome Shotgun Assembly Sequence Database (TSA).</p>
</li>
<li><p>Click where is says &quot;Limited by&quot; click organism and thereon specify the group of interest.</p>
</li>
</ol>
<p>Thats it.</p>

CC BY-SA 4.0
2023-12-18T16:10:25.983Z
The OP wants to do this "using command line" though. And I haven't managed to find a good way to do that, personally, which is why I came up with that [really complex workaround](https://bioinformatics.stackexchange.com/a/21806/298) for a similar question.
terdon
CC BY-SA 4.0
2023-12-18T15:00:16.317Z
2023-12-18T21:16:29.837Z
2023-12-18T21:16:29.837Z
M__
<p>Is there a way to blast my proteins against all the TSA (Transcriptome Shotgun Assembly Sequence Database) belonging to a specific taxonomy using command line?</p>
<p>Thanks,<br />
Marco</p>

CC BY-SA 4.0
2023-12-19T06:14:16.913Z
This seems like an [XY problem](https://en.wikipedia.org/wiki/XY_problem). Please add more detail to your question post, including information about the problem that you are trying to solve by doing this. Have you considered other approaches to discover the taxonomy of proteins?
gringer
<phylogenetics><phylogeny><proteins><taxonomy><blastn>
tBLASTn vs NCBI TSA
# tBLASTn vs NCBI TSA

(Asked by: username_0 on 2023-12-18)

Is there a way to blast my proteins against all the TSA (Transcriptome Shotgun Assembly Sequence Database) belonging to a specific taxonomy using command line?

Thanks,  
Marco

**Tags:** phylogenetics, phylogeny, proteins, taxonomy, blastn
> **Comments:**
> **username_1**: This seems like an [XY problem](https://en.wikipedia.org/wiki/XY_problem). Please add more detail to your question post, including information about the problem that you are trying to solve by doing this. Have you considered other approaches to discover the taxonomy of proteins?  

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## 1 answers

### Answer by username_2 on 2023-12-18. Score: 0

Just go to Blast [here](https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome)

1. Click where is says Database go to Transcriptome Shotgun Assembly Sequence Database (TSA).
2. Click where is says "Limited by" click organism and thereon specify the group of interest.

Thats it.

> **Comments:**
> **username_3**: The OP wants to do this "using command line" though. And I haven't managed to find a good way to do that, personally, which is why I came up with that [really complex workaround](https://bioinformatics.stackexchange.com/a/21806/298) for a similar question.  

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