<p>I found two methods:</p>
<ol>
<li>You want to visit this page for instructions for novel assemblies: <a href="http://genomewiki.ucsc.edu/index.php/Assembly_Hubs" rel="nofollow noreferrer">http://genomewiki.ucsc.edu/index.php/Assembly_Hubs</a> .</li>
<li>However, before doing that I highly recommend that you check Genome Assembly Hub first:
<a href="https://genome-test.gi.ucsc.edu/gbdb/hubs/genbank/" rel="nofollow noreferrer">https://genome-test.gi.ucsc.edu/gbdb/hubs/genbank/</a> . There is a good chance that your genome or strain is listed there. Here is an example of how you can do that. Example specifically shows how you can display E.coli BW25113 strain in the browser.</li>
</ol>
<hr />
<p><strong>Instructions:</strong></p>
<ol>
<li>Go to NCBI Nucleotide: <a href="https://www.ncbi.nlm.nih.gov/nucleotide" rel="nofollow noreferrer">https://www.ncbi.nlm.nih.gov/nucleotide</a> .</li>
<li>Enter your strain name: <code>E.coli BW25113 (GenBank: CP009273.1)</code></li>
<li>Find Biosample ID on the page, located under DBLINK: <code>BioSample: SAMN03013572</code></li>
<li>Go to GenBank assembly hubs: <a href="https://genome-test.gi.ucsc.edu/gbdb/hubs/genbank/" rel="nofollow noreferrer">https://genome-test.gi.ucsc.edu/gbdb/hubs/genbank/</a> .</li>
<li>Select bacteria. This will take you to &quot;Bacterial assembly hub&quot;
at <a href="https://genome-test.gi.ucsc.edu/gbdb/hubs/genbank/bacteria/bacteria.html" rel="nofollow noreferrer">https://genome-test.gi.ucsc.edu/gbdb/hubs/genbank/bacteria/bacteria.html</a> .</li>
<li>Look for <em>E. coli</em> hubs. At the time I looked #15-18 contain <em>E. coli</em> strains.</li>
<li>Click on each hub and search for <code>SAMN03013572</code> using browser search function (<kbd>Command</kbd>+<kbd>F</kbd> on macOS). I found my genome in hub #16.</li>
<li>Click on &quot;common name&quot; in the column-3. This will take you to
<a href="https://genome-test.gi.ucsc.edu/cgi-bin/hgGateway?hubUrl=http://genome-test.gi.ucsc.edu/gbdb/hubs/genbank/bacteria/16/hub.txt&amp;genome=GCA_000750555.1_ASM75055v1&amp;position=lastDbPos" rel="nofollow noreferrer">https://genome-test.gi.ucsc.edu/cgi-bin/hgGateway?hubUrl=http://genome-test.gi.ucsc.edu/gbdb/hubs/genbank/bacteria/16/hub.txt&amp;genome=GCA_000750555.1_ASM75055v1&amp;position=lastDbPos</a> , where <code>FindPosition</code> window will be populated with your genome Assembly.</li>
<li>Click on <kbd>Go</kbd>. This should take you to the UCSC Genome Browser with your genome assembly.</li>
</ol>

CC BY-SA 4.0
2022-11-24T01:52:26.967Z
Update: The step-9 is no longer working since UCSC genome browser resources described above were experimental prototype proof of concepts
that have since been discontinued according to the Genome Browser Team. Once I find an alternative method, I will post it here.
Supertech
CC BY-SA 4.0
2022-02-04T00:14:42.240Z
2022-02-04T13:49:50.310Z
2022-02-04T13:49:50.310Z
Supertech
<p>I am not sure if this qualifies as an official answer. I have contacted UCSC bioinformatics team. They were were very kind and told me that they would add any track that was requested by users. After my request, they kindly added the track for E.coli BW25113. If you want your reference tracks to be added you could submit a request at this website:
<a href="https://genome.ucsc.edu/assemblyRequest.html?all" rel="nofollow noreferrer">Geneme assembly search and request page.</a></p>
<p>Then, at the upper right corner click on &quot;Can't find your assembly?&quot; button and submit your request. See the screenshot below from the webpage.</p>
<p><a href="https://i.sstatic.net/Yacuh.png" rel="nofollow noreferrer"><img src="https://i.sstatic.net/Yacuh.png" alt="screenshot" /></a></p>

CC BY-SA 4.0
2022-12-09T01:42:45.753Z
2022-12-09T01:42:45.753Z
Supertech
<p>I want to display E.coli BW25113 (GenBank: CP009273.1) strain in UCSC browser. This strain is not listed in <a href="http://microbes.ucsc.edu/" rel="noreferrer">http://microbes.ucsc.edu/</a> browser. How can I display E.coli BW25113 assembly in the browser?</p>

<assembly><ucsc><bacteria><genome-browser>
How to display novel genome assemblies or uncommon genome assemblies using the UCSC Genome Browser?
# How to display novel genome assemblies or uncommon genome assemblies using the UCSC Genome Browser?

(Asked by: username_0 on 2022-02-04)

I want to display E.coli BW25113 (GenBank: CP009273.1) strain in UCSC browser. This strain is not listed in <http://microbes.ucsc.edu/> browser. How can I display E.coli BW25113 assembly in the browser?

**Tags:** assembly, ucsc, bacteria, genome-browser

---

## 2 answers

### Answer by username_0 on 2022-02-04. Score: 4

I found two methods:

1. You want to visit this page for instructions for novel assemblies: <http://genomewiki.ucsc.edu/index.php/Assembly_Hubs> .
2. However, before doing that I highly recommend that you check Genome Assembly Hub first:
   <https://genome-test.gi.ucsc.edu/gbdb/hubs/genbank/> . There is a good chance that your genome or strain is listed there. Here is an example of how you can do that. Example specifically shows how you can display E.coli BW25113 strain in the browser.

---

**Instructions:**

1. Go to NCBI Nucleotide: <https://www.ncbi.nlm.nih.gov/nucleotide> .
2. Enter your strain name: `E.coli BW25113 (GenBank: CP009273.1)`
3. Find Biosample ID on the page, located under DBLINK: `BioSample: SAMN03013572`
4. Go to GenBank assembly hubs: <https://genome-test.gi.ucsc.edu/gbdb/hubs/genbank/> .
5. Select bacteria. This will take you to "Bacterial assembly hub"
   at <https://genome-test.gi.ucsc.edu/gbdb/hubs/genbank/bacteria/bacteria.html> .
6. Look for *E. coli* hubs. At the time I looked #15-18 contain *E. coli* strains.
7. Click on each hub and search for `SAMN03013572` using browser search function (`Command`+`F` on macOS). I found my genome in hub #16.
8. Click on "common name" in the column-3. This will take you to
   <https://genome-test.gi.ucsc.edu/cgi-bin/hgGateway?hubUrl=http://genome-test.gi.ucsc.edu/gbdb/hubs/genbank/bacteria/16/hub.txt&genome=GCA_000750555.1_ASM75055v1&position=lastDbPos> , where `FindPosition` window will be populated with your genome Assembly.
9. Click on `Go`. This should take you to the UCSC Genome Browser with your genome assembly.

> **Comments:**
> **username_0**: Update: The step-9 is no longer working since UCSC genome browser resources described above were experimental prototype proof of concepts that have since been discontinued according to the Genome Browser Team. Once I find an alternative method, I will post it here.  

---

### Answer by username_0 on 2022-12-09. Score: 1

I am not sure if this qualifies as an official answer. I have contacted UCSC bioinformatics team. They were were very kind and told me that they would add any track that was requested by users. After my request, they kindly added the track for E.coli BW25113. If you want your reference tracks to be added you could submit a request at this website:
[Geneme assembly search and request page.](https://genome.ucsc.edu/assemblyRequest.html?all)

Then, at the upper right corner click on "Can't find your assembly?" button and submit your request. See the screenshot below from the webpage.

[![screenshot](https://i.sstatic.net/Yacuh.png)](https://i.sstatic.net/Yacuh.png)


---

