<p>It will not do any harm other than a reduction in statistical power compared to more samples per group. Common analysis tools such as DESeq2, edgeR and limma will work on unequal sample size just fine.</p>

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2023-03-01T17:48:53.430Z
2023-03-01T17:48:53.430Z
ATpoint
<p>I have 3 groups for the RNA-seq analysis (Control, treatment A and treatment B). There are 2 replicates for control and treatment A and 3 replicates for treatment B (lost 2 replicates due to a mistake in sample handling). May I ask how will the inconsistent replicate numbers impact the results (e.g. DEGs)?</p>

<rna-seq><genomics><differential-expression><gene-expression>
Inconsistent replicate numbers in RNA-seq
# Inconsistent replicate numbers in RNA-seq

(Asked by: username_0 on 2023-03-01)

I have 3 groups for the RNA-seq analysis (Control, treatment A and treatment B). There are 2 replicates for control and treatment A and 3 replicates for treatment B (lost 2 replicates due to a mistake in sample handling). May I ask how will the inconsistent replicate numbers impact the results (e.g. DEGs)?

**Tags:** rna-seq, genomics, differential-expression, gene-expression

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## 1 answers

### Answer by username_1 on 2023-03-01. Score: 2

It will not do any harm other than a reduction in statistical power compared to more samples per group. Common analysis tools such as DESeq2, edgeR and limma will work on unequal sample size just fine.


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