<p>Honestly, ask who made them.  If the reads are from an organism without splicing, it will not be trivial to figure it out with 100% certainty.</p>
<p>If you are calling variants, I don't think it matters much, except that higher organisms have such a small % of their genome transcribed, it should be obvious very quickly if you have the desired coverage across the entire genome, or just the transcripts.</p>

CC BY-SA 4.0
2021-03-10T20:33:53.050Z
2021-03-10T20:33:53.050Z
swbarnes2
<p>I have (gave me) Illumina fastq files, which I want to use for variant calling, and I do not know if they are DNAseq or RNAseq data. How can I check this? I do not have any report or who to ask.</p>
<p>Many thanks</p>

<rna-seq><phylogenetics><fastq>
Fastq: how can I check if they are from DNA or RNAseq data?
# Fastq: how can I check if they are from DNA or RNAseq data?

(Asked by: username_0 on 2021-03-10)

I have (gave me) Illumina fastq files, which I want to use for variant calling, and I do not know if they are DNAseq or RNAseq data. How can I check this? I do not have any report or who to ask.

Many thanks

**Tags:** rna-seq, phylogenetics, fastq

---

## 1 answers

### Answer by username_1 on 2021-03-10. Score: 4

Honestly, ask who made them. If the reads are from an organism without splicing, it will not be trivial to figure it out with 100% certainty.

If you are calling variants, I don't think it matters much, except that higher organisms have such a small % of their genome transcribed, it should be obvious very quickly if you have the desired coverage across the entire genome, or just the transcripts.


---

