<p>You are going to have to look up the library prep protocol used, and determine if it will capture miRNA.  My guess is, in the course of most RNASeq preps, small products are removed as contamination, so you won't have any real miRNA left behind.</p>
<p>I believe you need to use a special library prep designed for miRNA to study them.</p>

CC BY-SA 4.0
2022-08-29T16:59:11.753Z
2022-08-29T16:59:11.753Z
swbarnes2
<p>I am looking to perform expression analysis of miRNAs with normal RNA-seq data lacking small RNA-seq data?</p>
<p>Which path should I choose for known miRNAs and unknown new miRNAs?</p>
<p><strong>Data set:</strong> rna-seq data that analyzes the expression differences of a plant under stress.</p>
<p><strong>Objective</strong> To assess known and new miRNAs.</p>

CC BY-SA 4.0
2022-08-28T14:41:52.770Z
Please edit the question to limit it to a specific problem with enough detail to identify an adequate answer.
Community
CC BY-SA 4.0
2022-08-29T14:22:22.367Z
This looks a good question, outside my area. It may be helpful to provide further details of your study and data set and clarify what the `known miRNAs` represent.
M__
CC BY-SA 4.0
2022-08-29T16:43:37.177Z
There is rna-seq data that analyzes the expression differences of a plant under stress. From this data I would like to learn the expression profiles of known and new miRNAs.
Burak Muhammed ÖNER
<rna-seq><ngs><rna>
Expression analysis of miRNAs with normal RNA-seq data without small RNA-seq data
# Expression analysis of miRNAs with normal RNA-seq data without small RNA-seq data

(Asked by: username_0 on 2022-08-28)

I am looking to perform expression analysis of miRNAs with normal RNA-seq data lacking small RNA-seq data?

Which path should I choose for known miRNAs and unknown new miRNAs?

**Data set:** rna-seq data that analyzes the expression differences of a plant under stress.

**Objective** To assess known and new miRNAs.

**Tags:** rna-seq, ngs, rna
> **Comments:**
> **username_1**: Please edit the question to limit it to a specific problem with enough detail to identify an adequate answer.  
> **username_2**: This looks a good question, outside my area. It may be helpful to provide further details of your study and data set and clarify what the `known miRNAs` represent.  
> **username_0**: There is rna-seq data that analyzes the expression differences of a plant under stress. From this data I would like to learn the expression profiles of known and new miRNAs.  

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## 1 answers

### Answer by username_3 on 2022-08-29. Score: 1

You are going to have to look up the library prep protocol used, and determine if it will capture miRNA. My guess is, in the course of most RNASeq preps, small products are removed as contamination, so you won't have any real miRNA left behind.

I believe you need to use a special library prep designed for miRNA to study them.


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