<p>I've recently been pulling TLR genes for different species and keep running across an error called file.fa whenever I start pulling them. Whenever I pull the first gene it's fine and makes the file.fa but when I pull the second gene (TLR4) it comes up with a file.fa error and deleted the previous file.fa
made from when I pulled the first gene. Any feedback would be greatly appreciated.</p>
<pre><code>['NP_001273862', 'XP_006138208', 'XP_006138164', 'XP_014427015', 'XP_014429739']
Traceback (most recent call last):
  File &quot;blastp.py&quot;, line 104, in &lt;module&gt;
    SeqIO.write(record, &quot;file.fa&quot;, 'fasta')
  File &quot;/home/dylan_w190/miniconda3/lib/python3.8/site-packages/Bio/SeqIO/__init__.py&quot;, line 522, in write
    with as_handle(handle, &quot;w&quot;) as fp:
  File &quot;/home/dylan_w190/miniconda3/lib/python3.8/contextlib.py&quot;, line 113, in __enter__
    return next(self.gen)
  File &quot;/home/dylan_w190/miniconda3/lib/python3.8/site-packages/Bio/File.py&quot;, line 72, in as_handle
    with open(handleish, mode, **kwargs) as fp:
FileNotFoundError: [Errno 2] No such file or directory: 'file.fa'
</code></pre>

CC BY-SA 4.0
2021-02-13T07:48:48.433Z
Can U plz provide enough detail for me to try to reproduce the error?
Liam McIntyre
CC BY-SA 4.0
2021-02-13T17:13:51.807Z
Show your code please- the error is slightly counterintuitive to me since *writing* to a file should not lead to `FileNotFoundError`
Chris_Rands
<file-formats>
Not Able to pull genes due to file.fa error
# Not Able to pull genes due to file.fa error

(Asked by: username_0 on 2021-02-13)

I've recently been pulling TLR genes for different species and keep running across an error called file.fa whenever I start pulling them. Whenever I pull the first gene it's fine and makes the file.fa but when I pull the second gene (TLR4) it comes up with a file.fa error and deleted the previous file.fa
made from when I pulled the first gene. Any feedback would be greatly appreciated.
['NP_001273862', 'XP_006138208', 'XP_006138164', 'XP_014427015', 'XP_014429739']
Traceback (most recent call last):
  File "blastp.py", line 104, in 
    SeqIO.write(record, "file.fa", 'fasta')
  File "/home/dylan_w190/miniconda3/lib/python3.8/site-packages/Bio/SeqIO/__init__.py", line 522, in write
    with as_handle(handle, "w") as fp:
  File "/home/dylan_w190/miniconda3/lib/python3.8/contextlib.py", line 113, in __enter__
    return next(self.gen)
  File "/home/dylan_w190/miniconda3/lib/python3.8/site-packages/Bio/File.py", line 72, in as_handle
    with open(handleish, mode, **kwargs) as fp:
FileNotFoundError: [Errno 2] No such file or directory: 'file.fa'

**Tags:** file-formats
> **Comments:**
> **username_1**: Can U plz provide enough detail for me to try to reproduce the error?  
> **username_2**: Show your code please- the error is slightly counterintuitive to me since *writing* to a file should not lead to `FileNotFoundError`  

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## 0 answers

