<p>The length of the bars and their placement on the x-axis represents the length of the peptide and mapping to the source protein, respectively. The coloring represents the average difference in peptide MS intensity between the control and two experiments.</p>

CC BY-SA 4.0
2021-11-17T11:11:09.270Z
so i need the length information isnt it which is not given in that paper ..
kcm
CC BY-SA 4.0
2021-11-17T11:15:05.030Z
it can be done using ggplot i mean mapping the length of the peptide?
kcm
CC BY-SA 4.0
2021-11-17T12:56:48.037Z
Maybe I am not following your question, but you get the peptide lengths from the peptides sequences. What you are missing from the table you link to is the source protein sequences, HBb and Hba. The placement of the peptide in the source protein is your x value of the bars, and the length of the peptide is the height of the bar.
birey
CC BY-SA 4.0
2021-11-17T13:41:18.410Z
"What you are missing from the table you link to is the source protein sequences, HBb and Hba." yes this one . "The placement of the peptide in the source protein is your x value of the bars, and the length of the peptide is the height of the bar." color mapping is the zscore which i do have ..
kcm
CC BY-SA 4.0
2021-11-17T11:06:55.437Z
2021-11-17T11:06:55.437Z
birey
<p>I'm trying to replicate the figure given in this <a href="https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004085#pgen.1004085.s010" rel="nofollow noreferrer">paper</a> using this <a href="https://doi.org/10.1371/journal.pgen.1004085.s010" rel="nofollow noreferrer">table</a> which they have given.</p>
<p><a href="https://i.sstatic.net/xwABU.png" rel="nofollow noreferrer"><img src="https://i.sstatic.net/xwABU.png" alt="Figure 5" /></a></p>
<p>So what i understand from the figure is they have calculated the zscore between two condition and plotted the same.</p>
<p>Now How is the length of each bar which is the peptide here is variable is it the zscore or the actual peptide length?</p>
<p>Small subset of the data</p>
<pre><code>dput(head(d2))
structure(list(Peptide = structure(1:6, .Label = c(&quot;AAHLPAEFTPAVH&quot;, 
&quot;AAHLPAEFTPAVHASLD&quot;, &quot;AAWGKVG&quot;, &quot;AEFTPAVHA&quot;, &quot;AEFTPAVHASLDKFLASVSTVLTSKYR&quot;, 
&quot;AFSDGLAH&quot;, &quot;AFSDGLAHLD&quot;, &quot;AFSDGLAHLDNLK&quot;, &quot;AGEYG&quot;, &quot;AHAGEYG&quot;, 
&quot;AHAGEYGAEALE&quot;, &quot;AHAGEYGAEALERMF&quot;, &quot;AHAGEYGAEALERMFLSFPTTK&quot;, 
&quot;AHGKKVLG&quot;, &quot;AHLDNLK&quot;, &quot;AHLPAEFTPAVH&quot;, &quot;AHLPAEFTPAVHASLD&quot;, &quot;AHLPAEFTPAVHASLDKF&quot;, 
&quot;AHVDDMPNA&quot;, &quot;AHVDDMPNALS&quot;, &quot;ALSDLH&quot;, &quot;ALWGKVNVDEVG&quot;, &quot;ALWGKVNVDEVGGEA&quot;, 
&quot;ALWGKVNVDEVGGEALG&quot;, &quot;ALWGKVNVDEVGGEALGRLL&quot;, &quot;ANALAHKYH&quot;, &quot;ASLDKF&quot;, 
&quot;ASLDKFLA&quot;, &quot;ASLDKFLASVS&quot;, &quot;ASLDKFLASVSTVLT&quot;, &quot;ASLDKFLASVSTVLTS&quot;, 
&quot;ASLDKFLASVSTVLTSKYR&quot;, &quot;ATLS&quot;, &quot;AVAHVDD&quot;, &quot;AVAHVDDMPN&quot;, &quot;AVAHVDDMPNA&quot;, 
&quot;AVAHVDDMPNAL&quot;, &quot;AVAHVDDMPNALS&quot;, &quot;AVAHVDDMPNALSA&quot;, &quot;AYQKVVAGVANALAHK&quot;, 
&quot;DALT&quot;, &quot;DALTN&quot;, &quot;DDMPNA&quot;, &quot;DDMPNALS&quot;, &quot;DEVG&quot;, &quot;DEVGGEA&quot;, &quot;DEVGGEALG&quot;, 
&quot;DEVGGEALGRLL&quot;, &quot;DGLA&quot;, &quot;DGLAH&quot;, &quot;DGLAHLD&quot;, &quot;DGLAHLDNLK&quot;, &quot;DKLHVD&quot;, 
&quot;DKLHVDPEN&quot;, &quot;DKLHVDPENF&quot;, &quot;DKLHVDPENFR&quot;, &quot;DKLHVDPENFRL&quot;, &quot;DKLHVDPENFRLL&quot;, 
&quot;DKLHVDPENFRLLG&quot;, &quot;DKTNVK&quot;, &quot;DLH&quot;, &quot;DLS&quot;, &quot;DLSTPD&quot;, &quot;DLSTPDA&quot;, 
&quot;DMPNALS&quot;, &quot;DPENF&quot;, &quot;DPENFR&quot;, &quot;DPVNF&quot;, &quot;DPVNFK&quot;, &quot;EFTPAVH&quot;, &quot;EFTPAVHASLD&quot;, 
&quot;EFTPAVHASLDKFLASVSTVLTSKYR&quot;, &quot;EFTPPVQ&quot;, &quot;EFTPPVQA&quot;, &quot;EFTPPVQAA&quot;, 
&quot;ESFG&quot;, &quot;ESFGDL&quot;, &quot;ESFGDLSTPDAVM&quot;, &quot;ESFGDLSTPDAVMG&quot;, &quot;EVGG&quot;, 
&quot;EVGGEALG&quot;, &quot;FDLS&quot;, &quot;FESF&quot;, &quot;FESFG&quot;, &quot;FESFGDLS&quot;, &quot;FESFGDLSTPDAVMGNPKVK&quot;, 
&quot;FGDL&quot;, &quot;FGDLS&quot;, &quot;FPHF&quot;, &quot;FPHFD&quot;, &quot;FPHFDL&quot;, &quot;FPHFDLS&quot;, &quot;FPT&quot;, 
&quot;FPTT&quot;, &quot;FPTTKT&quot;, &quot;FPTTKTY&quot;, &quot;FPTTKTYFPHF&quot;, &quot;FSDGLA&quot;, &quot;FSDGLAH&quot;, 
&quot;FSDGLAHLD&quot;, &quot;FSDGLAHLDNLK&quot;, &quot;FTPAVH&quot;, &quot;FTPAVHASLD&quot;, &quot;FTPAVHASLDKFLASVSTVLT&quot;, 
&quot;FTPPVQ&quot;, &quot;FTPPVQA&quot;, &quot;FTPPVQAA&quot;, &quot;GAFSDGLA&quot;, &quot;GAFSDGLAH&quot;, &quot;GAFSDGLAHLDNLK&quot;, 
&quot;GEALG&quot;, &quot;GEYG&quot;, &quot;GGEAL&quot;, &quot;GKKVL&quot;, &quot;GKKVLG&quot;, &quot;GKVNVD&quot;, &quot;GKVNVDE&quot;, 
&quot;GKVNVDEVG&quot;, &quot;GKVNVDEVGGEA&quot;, &quot;GKVNVDEVGGEAL&quot;, &quot;GKVNVDEVGGEALG&quot;, 
&quot;GKVNVDEVGGEALGRL&quot;, &quot;GKVNVDEVGGEALGRLL&quot;, &quot;GLAHLDNLK&quot;, &quot;GNPKV&quot;, 
&quot;GNPKVK&quot;, &quot;GNVL&quot;, &quot;GRLL&quot;, &quot;GTFAT&quot;, &quot;GVANALAHKYH&quot;, &quot;HAGEYG&quot;, &quot;HAGEYGAEALE&quot;, 
&quot;HAGEYGAEALERMF&quot;, &quot;HFDLS&quot;, &quot;HHFG&quot;, &quot;HKYH&quot;, &quot;HLD&quot;, &quot;HLDNLK&quot;, &quot;HLPAE&quot;, 
&quot;HLPAEF&quot;, &quot;HLPAEFTPAVH&quot;, &quot;HLPAEFTPAVHASLD&quot;, &quot;HLTPEEK&quot;, &quot;HVDD&quot;, 
&quot;HVDDMP&quot;, &quot;HVDDMPN&quot;, &quot;HVDDMPNA&quot;, &quot;HVDDMPNAL&quot;, &quot;HVDDMPNALS&quot;, &quot;HVDDMPNALSA&quot;, 
&quot;HVDDMPNALSALS&quot;, &quot;HVDP&quot;, &quot;HVDPEN&quot;, &quot;HVDPENF&quot;, &quot;HVDPENFR&quot;, &quot;HVDPENFRL&quot;, 
&quot;HVDPENFRLLG&quot;, &quot;KAAWGKVG&quot;, &quot;KEFTPPVQ&quot;, &quot;KEFTPPVQA&quot;, &quot;KEFTPPVQAA&quot;, 
&quot;KEFTPPVQAAYQ&quot;, &quot;KLHVDPENFRLLG&quot;, &quot;KTYFP&quot;, &quot;KTYFPH&quot;, &quot;KTYFPHF&quot;, 
&quot;KTYFPHFD&quot;, &quot;KVNVDEVGG&quot;, &quot;KVNVDEVGGEA&quot;, &quot;KVNVDEVGGEALG&quot;, &quot;LDNL&quot;, 
&quot;LDNLK&quot;, &quot;LGAFSDGLA&quot;, &quot;LHVDP&quot;, &quot;LHVDPENF&quot;, &quot;LHVDPENFRLLG&quot;, &quot;LKGTFATL&quot;, 
&quot;LLVVYPWTQRF&quot;, &quot;LPAE&quot;, &quot;LPAEF&quot;, &quot;LPAEFTPAVH&quot;, &quot;LPAEFTPAVHASLD&quot;, 
&quot;LPAEFTPAVHASLDK&quot;, &quot;LPAEFTPAVHASLDKF&quot;, &quot;LSALS&quot;, &quot;LSFPTTK&quot;, &quot;LSFPTTKT&quot;, 
&quot;LSFPTTKTY&quot;, &quot;LSFPTTKTYFPHF&quot;, &quot;LSFPTTKTYFPHFDL&quot;, &quot;LSFPTTKTYFPHFDLS&quot;, 
&quot;LSFPTTKTYFPHFDLSHG&quot;, &quot;LSTPDA&quot;, &quot;LTPEEK&quot;, &quot;LTPEEKSAVT&quot;, &quot;LTPEEKSAVTA&quot;, 
&quot;LTPEEKSAVTAL&quot;, &quot;LTPEEKSAVTALW&quot;, &quot;LTPEEKSAVTALWG&quot;, &quot;LTPEEKSAVTALWGKVNVD&quot;, 
&quot;LTPEEKSAVTALWGKVNVDEVG&quot;, &quot;LTPEEKSAVTALWGKVNVDEVGGEA&quot;, &quot;LTPEEKSAVTALWGKVNVDEVGGEALG&quot;, 
&quot;LVVYPWTQRF&quot;, &quot;LVVYPWTQRFF&quot;, &quot;LWGKVNVDEVGGEALG&quot;, &quot;NAVAHVDD&quot;, 
&quot;NAVAHVDDMPNA&quot;, &quot;NAVAHVDDMPNAL&quot;, &quot;NAVAHVDDMPNALS&quot;, &quot;NAVAHVDDMPNALSA&quot;, 
&quot;NAVAHVDDMPNALSALS&quot;, &quot;NAVAHVDDMPNALSALSDL&quot;, &quot;NAVAHVDDMPNALSALSDLHAHKLR&quot;, 
&quot;NVDEVG&quot;, &quot;NVDEVGG&quot;, &quot;NVDEVGGEA&quot;, &quot;NVDEVGGEALG&quot;, &quot;PADKTNVK&quot;, 
&quot;PAEF&quot;, &quot;PAEFTPAVH&quot;, &quot;PAEFTPAVHASLD&quot;, &quot;PAVH&quot;, &quot;PDAV&quot;, &quot;PDAVM&quot;, 
&quot;PDAVMG&quot;, &quot;PEE&quot;, &quot;PEEK&quot;, &quot;PEEKSAVT&quot;, &quot;PEEKSAVTAL&quot;, &quot;PENF&quot;, &quot;PENFR&quot;, 
&quot;PENFRLLG&quot;, &quot;PHF&quot;, &quot;PHFD&quot;, &quot;PHFDL&quot;, &quot;PHFDLS&quot;, &quot;PKVK&quot;, &quot;PNAL&quot;, 
&quot;PNALS&quot;, &quot;PPVQ&quot;, &quot;PPVQA&quot;, &quot;PPVQAA&quot;, &quot;PVNF&quot;, &quot;PVNFK&quot;, &quot;PVQ&quot;, &quot;PVQA&quot;, 
&quot;PWTQ&quot;, &quot;PWTQRF&quot;, &quot;PWTQRFF&quot;, &quot;QVKGHGKKVADALTN&quot;, &quot;RVDPVNF&quot;, &quot;SDGLA&quot;, 
&quot;SDGLAHLDN&quot;, &quot;SDGLAHLDNLK&quot;, &quot;SDLH&quot;, &quot;SFGD&quot;, &quot;SFGDL&quot;, &quot;SFGDLS&quot;, 
&quot;SFGDLSTPDAVMG&quot;, &quot;SLDKF&quot;, &quot;SPADK&quot;, &quot;SPADKTNVK&quot;, &quot;SVSTVLT&quot;, &quot;TKTYFPHF&quot;, 
&quot;TNVK&quot;, &quot;TPAVH&quot;, &quot;TPAVHASLD&quot;, &quot;TPAVHASLDKFLASVSTVLT&quot;, &quot;TPDAVM&quot;, 
&quot;TPDAVMG&quot;, &quot;TPDAVMGNPKVK&quot;, &quot;TPEEKSAVT&quot;, &quot;TPEEKSAVTAL&quot;, &quot;TPEEKSAVTALW&quot;, 
&quot;TPPVQ&quot;, &quot;TSKY&quot;, &quot;TVLT&quot;, &quot;TYFP&quot;, &quot;TYFPH&quot;, &quot;TYFPHF&quot;, &quot;TYFPHFD&quot;, 
&quot;TYFPHFDLS&quot;, &quot;VAHVDD&quot;, &quot;VAHVDDMPNA&quot;, &quot;VAHVDDMPNALS&quot;, &quot;VAHVDDMPNALSA&quot;, 
&quot;VDDMP&quot;, &quot;VDDMPN&quot;, &quot;VDDMPNA&quot;, &quot;VDDMPNAL&quot;, &quot;VDDMPNALS&quot;, &quot;VDEVG&quot;, 
&quot;VDEVGG&quot;, &quot;VDEVGGEA&quot;, &quot;VDEVGGEALG&quot;, &quot;VDPENF&quot;, &quot;VDPENFR&quot;, &quot;VDPENFRL&quot;, 
&quot;VDPENFRLL&quot;, &quot;VDPENFRLLG&quot;, &quot;VDPVN&quot;, &quot;VDPVNF&quot;, &quot;VDPVNFK&quot;, &quot;VDPVNFKL&quot;, 
&quot;VDPVNFKLL&quot;, &quot;VDPVNFKLLS&quot;, &quot;VHL&quot;, &quot;VHLT&quot;, &quot;VHLTP&quot;, &quot;VHLTPE&quot;, 
&quot;VHLTPEE&quot;, &quot;VHLTPEEK&quot;, &quot;VHLTPEEKSAVT&quot;, &quot;VHLTPEEKSAVTAL&quot;, &quot;VHLTPEEKSAVTALWGKVNVD&quot;, 
&quot;VHLTPEEKSAVTALWGKVNVDE&quot;, &quot;VHLTPEEKSAVTALWGKVNVDEVG&quot;, &quot;VHLTPEEKSAVTALWGKVNVDEVGGEA&quot;, 
&quot;VHLTPEEKSAVTALWGKVNVDEVGGEALG&quot;, &quot;VHLTPEEKSAVTALWGKVNVDEVGGEALGRLL&quot;, 
&quot;VKAAWGKVGAHAGEY&quot;, &quot;VLSP&quot;, &quot;VLSPA&quot;, &quot;VLSPAD&quot;, &quot;VLSPADK&quot;, &quot;VLSPADKT&quot;, 
&quot;VLSPADKTN&quot;, &quot;VLSPADKTNVK&quot;, &quot;VLSPADKTNVKAA&quot;, &quot;VLSPADKTNVKAAWGKVG&quot;, 
&quot;VNVDE&quot;, &quot;VNVDEVG&quot;, &quot;VNVDEVGG&quot;, &quot;VNVDEVGGEA&quot;, &quot;VNVDEVGGEAL&quot;, 
&quot;VNVDEVGGEALG&quot;, &quot;VNVDEVGGEALGRL&quot;, &quot;VVAGVANALA&quot;, &quot;VVYPWTQRF&quot;, 
&quot;VVYPWTQRFF&quot;, &quot;VVYPWTQRFFE&quot;, &quot;VVYPWTQRFFESFGDLS&quot;, &quot;VYPWTQRF&quot;, 
&quot;VYPWTQRFF&quot;, &quot;VYPWTQRFFE&quot;, &quot;WGKVG&quot;, &quot;WGKVNVD&quot;, &quot;WGKVNVDE&quot;, &quot;WGKVNVDEVG&quot;, 
&quot;WGKVNVDEVGGEA&quot;, &quot;WGKVNVDEVGGEAL&quot;, &quot;WGKVNVDEVGGEALG&quot;, &quot;WGKVNVDEVGGEALGRL&quot;, 
&quot;WGKVNVDEVGGEALGRLL&quot;, &quot;YFPHFDLS&quot;, &quot;YPWT&quot;, &quot;YPWTQ&quot;, &quot;YPWTQR&quot;, 
&quot;YPWTQRF&quot;, &quot;YPWTQRFF&quot;, &quot;YPWTQRFFE&quot;), class = &quot;factor&quot;), C2 = c(-1.13005927266738, 
-1.15451123584162, -0.771975424114207, -0.891503793435265, 0.686928914375861, 
-1.15469327326147), C4 = c(0.770515719059141, 0.559148858503814, 
1.12965461223673, 1.08129555353272, 0.46033682419062, 0.58089396147208
), C6 = c(0.359543553608241, 0.595362377337809, -0.357679188122518, 
-0.18979176009746, -1.14726573856648, 0.573799311789392)), row.names = c(NA, 
6L), class = &quot;data.frame&quot;)
</code></pre>
<p>Any suggestion or help would be really appreciated</p>

<visualization><proteomics>
Peptide data visualization
# Peptide data visualization

(Asked by: username_0 on 2021-11-17)

I'm trying to replicate the figure given in this paper using this table which they have given.

So what i understand from the figure is they have calculated the zscore between two condition and plotted the same.
Now How is the length of each bar which is the peptide here is variable is it the zscore or the actual peptide length?
Small subset of the data
dput(head(d2))
structure(list(Peptide = structure(1:6, .Label = c("AAHLPAEFTPAVH", 
"AAHLPAEFTPAVHASLD", "AAWGKVG", "AEFTPAVHA", "AEFTPAVHASLDKFLASVSTVLTSKYR", 
"AFSDGLAH", "AFSDGLAHLD", "AFSDGLAHLDNLK", "AGEYG", "AHAGEYG", 
"AHAGEYGAEALE", "AHAGEYGAEALERMF", "AHAGEYGAEALERMFLSFPTTK", 
"AHGKKVLG", "AHLDNLK", "AHLPAEFTPAVH", "AHLPAEFTPAVHASLD", "AHLPAEFTPAVHASLDKF", 
"AHVDDMPNA", "AHVDDMPNALS", "ALSDLH", "ALWGKVNVDEVG", "ALWGKVNVDEVGGEA", 
"ALWGKVNVDEVGGEALG", "ALWGKVNVDEVGGEALGRLL", "ANALAHKYH", "ASLDKF", 
"ASLDKFLA", "ASLDKFLASVS", "ASLDKFLASVSTVLT", "ASLDKFLASVSTVLTS", 
"ASLDKFLASVSTVLTSKYR", "ATLS", "AVAHVDD", "AVAHVDDMPN", "AVAHVDDMPNA", 
"AVAHVDDMPNAL", "AVAHVDDMPNALS", "AVAHVDDMPNALSA", "AYQKVVAGVANALAHK", 
"DALT", "DALTN", "DDMPNA", "DDMPNALS", "DEVG", "DEVGGEA", "DEVGGEALG", 
"DEVGGEALGRLL", "DGLA", "DGLAH", "DGLAHLD", "DGLAHLDNLK", "DKLHVD", 
"DKLHVDPEN", "DKLHVDPENF", "DKLHVDPENFR", "DKLHVDPENFRL", "DKLHVDPENFRLL", 
"DKLHVDPENFRLLG", "DKTNVK", "DLH", "DLS", "DLSTPD", "DLSTPDA", 
"DMPNALS", "DPENF", "DPENFR", "DPVNF", "DPVNFK", "EFTPAVH", "EFTPAVHASLD", 
"EFTPAVHASLDKFLASVSTVLTSKYR", "EFTPPVQ", "EFTPPVQA", "EFTPPVQAA", 
"ESFG", "ESFGDL", "ESFGDLSTPDAVM", "ESFGDLSTPDAVMG", "EVGG", 
"EVGGEALG", "FDLS", "FESF", "FESFG", "FESFGDLS", "FESFGDLSTPDAVMGNPKVK", 
"FGDL", "FGDLS", "FPHF", "FPHFD", "FPHFDL", "FPHFDLS", "FPT", 
"FPTT", "FPTTKT", "FPTTKTY", "FPTTKTYFPHF", "FSDGLA", "FSDGLAH", 
"FSDGLAHLD", "FSDGLAHLDNLK", "FTPAVH", "FTPAVHASLD", "FTPAVHASLDKFLASVSTVLT", 
"FTPPVQ", "FTPPVQA", "FTPPVQAA", "GAFSDGLA", "GAFSDGLAH", "GAFSDGLAHLDNLK", 
"GEALG", "GEYG", "GGEAL", "GKKVL", "GKKVLG", "GKVNVD", "GKVNVDE", 
"GKVNVDEVG", "GKVNVDEVGGEA", "GKVNVDEVGGEAL", "GKVNVDEVGGEALG", 
"GKVNVDEVGGEALGRL", "GKVNVDEVGGEALGRLL", "GLAHLDNLK", "GNPKV", 
"GNPKVK", "GNVL", "GRLL", "GTFAT", "GVANALAHKYH", "HAGEYG", "HAGEYGAEALE", 
"HAGEYGAEALERMF", "HFDLS", "HHFG", "HKYH", "HLD", "HLDNLK", "HLPAE", 
"HLPAEF", "HLPAEFTPAVH", "HLPAEFTPAVHASLD", "HLTPEEK", "HVDD", 
"HVDDMP", "HVDDMPN", "HVDDMPNA", "HVDDMPNAL", "HVDDMPNALS", "HVDDMPNALSA", 
"HVDDMPNALSALS", "HVDP", "HVDPEN", "HVDPENF", "HVDPENFR", "HVDPENFRL", 
"HVDPENFRLLG", "KAAWGKVG", "KEFTPPVQ", "KEFTPPVQA", "KEFTPPVQAA", 
"KEFTPPVQAAYQ", "KLHVDPENFRLLG", "KTYFP", "KTYFPH", "KTYFPHF", 
"KTYFPHFD", "KVNVDEVGG", "KVNVDEVGGEA", "KVNVDEVGGEALG", "LDNL", 
"LDNLK", "LGAFSDGLA", "LHVDP", "LHVDPENF", "LHVDPENFRLLG", "LKGTFATL", 
"LLVVYPWTQRF", "LPAE", "LPAEF", "LPAEFTPAVH", "LPAEFTPAVHASLD", 
"LPAEFTPAVHASLDK", "LPAEFTPAVHASLDKF", "LSALS", "LSFPTTK", "LSFPTTKT", 
"LSFPTTKTY", "LSFPTTKTYFPHF", "LSFPTTKTYFPHFDL", "LSFPTTKTYFPHFDLS", 
"LSFPTTKTYFPHFDLSHG", "LSTPDA", "LTPEEK", "LTPEEKSAVT", "LTPEEKSAVTA", 
"LTPEEKSAVTAL", "LTPEEKSAVTALW", "LTPEEKSAVTALWG", "LTPEEKSAVTALWGKVNVD", 
"LTPEEKSAVTALWGKVNVDEVG", "LTPEEKSAVTALWGKVNVDEVGGEA", "LTPEEKSAVTALWGKVNVDEVGGEALG", 
"LVVYPWTQRF", "LVVYPWTQRFF", "LWGKVNVDEVGGEALG", "NAVAHVDD", 
"NAVAHVDDMPNA", "NAVAHVDDMPNAL", "NAVAHVDDMPNALS", "NAVAHVDDMPNALSA", 
"NAVAHVDDMPNALSALS", "NAVAHVDDMPNALSALSDL", "NAVAHVDDMPNALSALSDLHAHKLR", 
"NVDEVG", "NVDEVGG", "NVDEVGGEA", "NVDEVGGEALG", "PADKTNVK", 
"PAEF", "PAEFTPAVH", "PAEFTPAVHASLD", "PAVH", "PDAV", "PDAVM", 
"PDAVMG", "PEE", "PEEK", "PEEKSAVT", "PEEKSAVTAL", "PENF", "PENFR", 
"PENFRLLG", "PHF", "PHFD", "PHFDL", "PHFDLS", "PKVK", "PNAL", 
"PNALS", "PPVQ", "PPVQA", "PPVQAA", "PVNF", "PVNFK", "PVQ", "PVQA", 
"PWTQ", "PWTQRF", "PWTQRFF", "QVKGHGKKVADALTN", "RVDPVNF", "SDGLA", 
"SDGLAHLDN", "SDGLAHLDNLK", "SDLH", "SFGD", "SFGDL", "SFGDLS", 
"SFGDLSTPDAVMG", "SLDKF", "SPADK", "SPADKTNVK", "SVSTVLT", "TKTYFPHF", 
"TNVK", "TPAVH", "TPAVHASLD", "TPAVHASLDKFLASVSTVLT", "TPDAVM", 
"TPDAVMG", "TPDAVMGNPKVK", "TPEEKSAVT", "TPEEKSAVTAL", "TPEEKSAVTALW", 
"TPPVQ", "TSKY", "TVLT", "TYFP", "TYFPH", "TYFPHF", "TYFPHFD", 
"TYFPHFDLS", "VAHVDD", "VAHVDDMPNA", "VAHVDDMPNALS", "VAHVDDMPNALSA", 
"VDDMP", "VDDMPN", "VDDMPNA", "VDDMPNAL", "VDDMPNALS", "VDEVG", 
"VDEVGG", "VDEVGGEA", "VDEVGGEALG", "VDPENF", "VDPENFR", "VDPENFRL", 
"VDPENFRLL", "VDPENFRLLG", "VDPVN", "VDPVNF", "VDPVNFK", "VDPVNFKL", 
"VDPVNFKLL", "VDPVNFKLLS", "VHL", "VHLT", "VHLTP", "VHLTPE", 
"VHLTPEE", "VHLTPEEK", "VHLTPEEKSAVT", "VHLTPEEKSAVTAL", "VHLTPEEKSAVTALWGKVNVD", 
"VHLTPEEKSAVTALWGKVNVDE", "VHLTPEEKSAVTALWGKVNVDEVG", "VHLTPEEKSAVTALWGKVNVDEVGGEA", 
"VHLTPEEKSAVTALWGKVNVDEVGGEALG", "VHLTPEEKSAVTALWGKVNVDEVGGEALGRLL", 
"VKAAWGKVGAHAGEY", "VLSP", "VLSPA", "VLSPAD", "VLSPADK", "VLSPADKT", 
"VLSPADKTN", "VLSPADKTNVK", "VLSPADKTNVKAA", "VLSPADKTNVKAAWGKVG", 
"VNVDE", "VNVDEVG", "VNVDEVGG", "VNVDEVGGEA", "VNVDEVGGEAL", 
"VNVDEVGGEALG", "VNVDEVGGEALGRL", "VVAGVANALA", "VVYPWTQRF", 
"VVYPWTQRFF", "VVYPWTQRFFE", "VVYPWTQRFFESFGDLS", "VYPWTQRF", 
"VYPWTQRFF", "VYPWTQRFFE", "WGKVG", "WGKVNVD", "WGKVNVDE", "WGKVNVDEVG", 
"WGKVNVDEVGGEA", "WGKVNVDEVGGEAL", "WGKVNVDEVGGEALG", "WGKVNVDEVGGEALGRL", 
"WGKVNVDEVGGEALGRLL", "YFPHFDLS", "YPWT", "YPWTQ", "YPWTQR", 
"YPWTQRF", "YPWTQRFF", "YPWTQRFFE"), class = "factor"), C2 = c(-1.13005927266738, 
-1.15451123584162, -0.771975424114207, -0.891503793435265, 0.686928914375861, 
-1.15469327326147), C4 = c(0.770515719059141, 0.559148858503814, 
1.12965461223673, 1.08129555353272, 0.46033682419062, 0.58089396147208
), C6 = c(0.359543553608241, 0.595362377337809, -0.357679188122518, 
-0.18979176009746, -1.14726573856648, 0.573799311789392)), row.names = c(NA, 
6L), class = "data.frame")

Any suggestion or help would be really appreciated

**Tags:** visualization, proteomics

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## 1 answers

### Answer by username_1 on 2021-11-17. Score: 2 | Accepted answer

The length of the bars and their placement on the x-axis represents the length of the peptide and mapping to the source protein, respectively. The coloring represents the average difference in peptide MS intensity between the control and two experiments.

> **Comments:**
> **username_0**: so i need the length information isnt it which is not given in that paper ..  
> **username_0**: it can be done using ggplot i mean mapping the length of the peptide?  
> **username_1**: Maybe I am not following your question, but you get the peptide lengths from the peptides sequences. What you are missing from the table you link to is the source protein sequences, HBb and Hba. The placement of the peptide in the source protein is your x value of the bars, and the length of the peptide is the height of the bar.  
> **username_0**: "What you are missing from the table you link to is the source protein sequences, HBb and Hba." yes this one . "The placement of the peptide in the source protein is your x value of the bars, and the length of the peptide is the height of the bar." color mapping is the zscore which i do have ..  

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