<p>Yes, you can stop the run and it should not affect the base calling. We normally do the runs and then do the base calling after the run has completed.</p>

CC BY-SA 4.0
2018-10-26T20:08:33.717Z
2018-10-26T20:08:33.717Z
Saidi Rahina Hussain
<p>Yes, you can click "stop acquisition" and your run won't be negatively affected. All of the reads are saved as they are generated. I am not sure how this will impact live basecalling though if that is something that you do.</p>

CC BY-SA 3.0
2018-03-04T08:06:26.763Z
Live basecalling for the uncalled reads will continue until all have been called, or the application is quit.
gringer
CC BY-SA 3.0
2018-03-04T03:00:14.297Z
2018-03-04T03:00:14.297Z
conchoecia
<p>I am sequencing a whole genome on MinION. I have used this flow cell for 6 hours for the phage lambda control. That gave me around 300,000 reads. I started a 48h sequencing run on the same flow cell after washing it. The sequencing has been going on for 1 day and 21 hours. It is not producing any reads at the moment (Strand = 0). The number of single pores is also 0. Can I "Stop acquisition" without negatively affecting the results?</p>

<p>The image below is a screenshot taken from MinKNOW, the software used to control the MinION.</p>

<p><a href="https://i.sstatic.net/D5FuH.png" rel="nofollow noreferrer"><img src="https://i.sstatic.net/D5FuH.png" alt="Pore description and read summary"></a></p>

<nanopore><ngs><minion><long-reads><minknow>
Can I stop my nanopore sequencing run if there are no more reads being produced?
# Can I stop my nanopore sequencing run if there are no more reads being produced?

(Asked by: username_0 on 2018-03-03)

I am sequencing a whole genome on MinION. I have used this flow cell for 6 hours for the phage lambda control. That gave me around 300,000 reads. I started a 48h sequencing run on the same flow cell after washing it. The sequencing has been going on for 1 day and 21 hours. It is not producing any reads at the moment (Strand = 0). The number of single pores is also 0. Can I "Stop acquisition" without negatively affecting the results?

The image below is a screenshot taken from MinKNOW, the software used to control the MinION.

[![Pore description and read summary](https://i.sstatic.net/D5FuH.png)](https://i.sstatic.net/D5FuH.png)

**Tags:** nanopore, ngs, minion, long-reads, minknow

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## 2 answers

### Answer by username_2 on 2018-03-04. Score: 6 | Accepted answer

Yes, you can click "stop acquisition" and your run won't be negatively affected. All of the reads are saved as they are generated. I am not sure how this will impact live basecalling though if that is something that you do.

> **Comments:**
> **username_3**: Live basecalling for the uncalled reads will continue until all have been called, or the application is quit.  

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### Answer by username_1 on 2018-10-26. Score: 1

Yes, you can stop the run and it should not affect the base calling. We normally do the runs and then do the base calling after the run has completed.


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