<p>I want to find genes of fundamental metabolic processes in the <em>Phytophthora cinnamomi</em> genome. I'm using the genome sequence deposited in the NCBI and I'm searching ORF by ORF, using the ORF finder (<a href="https://www.ncbi.nlm.nih.gov/orffinder/" rel="nofollow noreferrer">https://www.ncbi.nlm.nih.gov/orffinder/</a>). Next, I use SMARTBLAST (<a href="https://blast.ncbi.nlm.nih.gov/smartblast/?LINK_LOC=BlastHomeLink" rel="nofollow noreferrer">https://blast.ncbi.nlm.nih.gov/smartblast/?LINK_LOC=BlastHomeLink</a>) to find similar organisms. 
How do I know that the gene that I'm searching expresses proteins of fundamental metabolic processes, and if the organism is similar to mine?
Can the best hits in SMARTBLAST tell me something about that?
Thanks</p>

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2020-02-16T19:55:49.840Z
Should be a bit quiter now ;)
finswimmer
<phylogenetics><gene><blast><sequence-homology>
How can I find genes of fundamental metabolic processes for an organism?
# How can I find genes of fundamental metabolic processes for an organism?

(Asked by: username_0 on 2020-02-15)

I want to find genes of fundamental metabolic processes in the *Phytophthora cinnamomi* genome. I'm using the genome sequence deposited in the NCBI and I'm searching ORF by ORF, using the ORF finder (<https://www.ncbi.nlm.nih.gov/orffinder/>). Next, I use SMARTBLAST (<https://blast.ncbi.nlm.nih.gov/smartblast/?LINK_LOC=BlastHomeLink>) to find similar organisms.
How do I know that the gene that I'm searching expresses proteins of fundamental metabolic processes, and if the organism is similar to mine?
Can the best hits in SMARTBLAST tell me something about that?
Thanks

**Tags:** phylogenetics, gene, blast, sequence-homology
> **Comments:**
> **username_1**: Should be a bit quiter now ;)  

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## 0 answers

