<p>I am trying to understand expression of a certain protein across Pseudomonas species. I downloaded an SRA file from NCBI and converted it to a fastaq file. I am not able to understand how to interpret and analyse this file. I want to get the mRNA sequence and see which genes are being expressed and find the mRNA of interest.</p>

<p>How do I proceed with getting the mRNA sequences and then for mapping them to genome?</p>

CC BY-SA 4.0
2020-05-12T14:54:29.210Z
For some studies, in GEO they also include the processed counts. You can also see whether it's the case for your study
StupidWolf
<rna-seq><rna><transcriptome><gene-expression>
How to understand and analyse RNA-seq data (for a beginner)?
# How to understand and analyse RNA-seq data (for a beginner)?

(Asked by: username_0 on 2020-05-12)

I am trying to understand expression of a certain protein across Pseudomonas species. I downloaded an SRA file from NCBI and converted it to a fastaq file. I am not able to understand how to interpret and analyse this file. I want to get the mRNA sequence and see which genes are being expressed and find the mRNA of interest.

How do I proceed with getting the mRNA sequences and then for mapping them to genome?

**Tags:** rna-seq, rna, transcriptome, gene-expression
> **Comments:**
> **username_1**: For some studies, in GEO they also include the processed counts. You can also see whether it's the case for your study  

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## 0 answers

