<p>I don't know of any online tool enabling a search against TrEMBL. You probably will have to set up your own local BLAST environment, roughly following these steps:</p>

<ol>
<li><a href="https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&amp;PAGE_TYPE=BlastDocs&amp;DOC_TYPE=Download" rel="nofollow noreferrer">download blast+</a> - but there might be an easier way to install the software, depending on your operating system (e.g. on Ubuntu you can just <code>sudo apt install ncbi-blast+</code>)</li>
<li><a href="https://www.uniprot.org/downloads" rel="nofollow noreferrer">download target databases</a></li>
<li><a href="https://www.ncbi.nlm.nih.gov/books/NBK279688/" rel="nofollow noreferrer">build local database</a></li>
</ol>

<p>I hope this helps.</p>

CC BY-SA 4.0
2020-04-25T20:58:54.317Z
thank you a lot for your answer!unfortunately my programming skills are not so strong!
marilu
CC BY-SA 4.0
2020-04-25T15:31:23.230Z
2020-04-25T15:31:23.230Z
mrhd
<p>UniProt provides a BLAST service (<a href="https://www.uniprot.org/blast" rel="nofollow noreferrer">https://www.uniprot.org/blast</a>) which you can run against several UniProt databases, or you could use the Expasy BLAST too, at <a href="https://web.expasy.org/blast" rel="nofollow noreferrer">https://web.expasy.org/blast</a>.</p>

CC BY-SA 4.0
2020-10-23T15:52:04.287Z
2020-10-23T15:52:04.287Z
Elisabeth.Gasteiger
<p>i need to search for one protein against UNIPROT in BLAST for a selected organism.
How can I do it? (SWSISSPROT+TREMBL)
THANKS!!</p>

<blast><uniprot>
how can i search in p-blast against UNIPROT(not only swissprot)
# how can i search in p-blast against UNIPROT(not only swissprot)

(Asked by: username_0 on 2020-04-25)

i need to search for one protein against UNIPROT in BLAST for a selected organism.
How can I do it? (SWSISSPROT+TREMBL)
THANKS!!

**Tags:** blast, uniprot

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## 2 answers

### Answer by username_1 on 2020-04-25. Score: 1 | Accepted answer

I don't know of any online tool enabling a search against TrEMBL. You probably will have to set up your own local BLAST environment, roughly following these steps:

1. [download blast+](https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE_TYPE=BlastDocs&DOC_TYPE=Download) - but there might be an easier way to install the software, depending on your operating system (e.g. on Ubuntu you can just `sudo apt install ncbi-blast+`)
2. [download target databases](https://www.uniprot.org/downloads)
3. [build local database](https://www.ncbi.nlm.nih.gov/books/NBK279688/)

I hope this helps.

> **Comments:**
> **username_0**: thank you a lot for your answer!unfortunately my programming skills are not so strong!  

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### Answer by username_2 on 2020-10-23. Score: 0

UniProt provides a BLAST service (<https://www.uniprot.org/blast>) which you can run against several UniProt databases, or you could use the Expasy BLAST too, at <https://web.expasy.org/blast>.


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