<p>I want to identify the <strong>most highly expressed genes across several developmental stages of a given insect</strong>, in hopes of identifying a protein that can be used to design an insecticide.
I have a few doubts :</p>
<p>Objective</p>
<ul>
<li>Several studies have been performed on <strong>individual developmental stages</strong> and their transcriptome has been published. <strong>Should I compare each set of data against the other manually</strong>, to find genes that are expressed universally and then target the most highly expressed ones or is there any <strong>software / computational method that can be used instead</strong>?</li>
</ul>
<p>Question</p>
<p><strong>Is it right to compare transcriptomes to find commonly expressed genes?</strong></p>
<p>I did try to look at the materials and methods section of a few papers on comparative transcriptomics, but it only says that data was compared without stating the method. One paper cited <code>VennBLAST</code>, but I wasn't able to find the tool online.</p>

CC BY-SA 3.0
2018-01-04T17:08:53.033Z
Could you clarify what do you mean by individual developmental stages? They looked at the same individual in several stages or they look in several samples different stages of development. What I think you want to do is find differentially expressed genes, search for those terms and let us know if you need something else
llrs
CC BY-SA 3.0
2018-01-04T17:24:06.560Z
I mean that each paper concentrates on a single developmental stage. They analyze several samples for different stages of development.

Also, no, I do not want to find differentially expressed genes.From what I understand, differentially expressed genes are those that are upregulated only in a particular cell type (in my case stage). I want to find out if a common set of genes are being upregulated throughout the life cycle of the insect, ie. they are common to all the life stages. I thought I can do this by analyzing the transcriptomes of each stage. I hope this is clear!
Cookie-san
CC BY-SA 3.0
2018-01-04T17:38:40.803Z
You can find the differentially expressed genes that are downregulated too, and it is not specific to a cell type that depends on your data. But it is a comparison between two stages, so you can compare all the initial stage with all the following stages. Then you find the common genes that are up-regulated in all the cases of interest
llrs
CC BY-SA 3.0
2018-01-04T17:44:58.640Z
Oh! I didn't know that differentially downregulated genes can be found too! Thanks for your answer :D
Cookie-san
CC BY-SA 3.0
2018-01-04T18:17:38.620Z
This was a comment!! You seem to need more guidance, ask your advisor or a senior people how to find the most expressed genes and from there how to find it those that are so in several stages.
llrs
<transcriptome>
How do I compare transcriptomes of the same species across different developmental stages?
# How do I compare transcriptomes of the same species across different developmental stages?

(Asked by: username_0 on 2018-01-04)

I want to identify the **most highly expressed genes across several developmental stages of a given insect**, in hopes of identifying a protein that can be used to design an insecticide.
I have a few doubts :

Objective

* Several studies have been performed on **individual developmental stages** and their transcriptome has been published. **Should I compare each set of data against the other manually**, to find genes that are expressed universally and then target the most highly expressed ones or is there any **software / computational method that can be used instead**?

Question

**Is it right to compare transcriptomes to find commonly expressed genes?**

I did try to look at the materials and methods section of a few papers on comparative transcriptomics, but it only says that data was compared without stating the method. One paper cited `VennBLAST`, but I wasn't able to find the tool online.

**Tags:** transcriptome
> **Comments:**
> **username_1**: Could you clarify what do you mean by individual developmental stages? They looked at the same individual in several stages or they look in several samples different stages of development. What I think you want to do is find differentially expressed genes, search for those terms and let us know if you need something else  
> **username_0**: I mean that each paper concentrates on a single developmental stage. They analyze several samples for different stages of development.  Also, no, I do not want to find differentially expressed genes.From what I understand, differentially expressed genes are those that are upregulated only in a particular cell type (in my case stage). I want to find out if a common set of genes are being upregulated throughout the life cycle of the insect, ie. they are common to all the life stages. I thought I can do this by analyzing the transcriptomes of each stage. I hope this is clear!  
> **username_1**: You can find the differentially expressed genes that are downregulated too, and it is not specific to a cell type that depends on your data. But it is a comparison between two stages, so you can compare all the initial stage with all the following stages. Then you find the common genes that are up-regulated in all the cases of interest  
> **username_0**: Oh! I didn't know that differentially downregulated genes can be found too! Thanks for your answer :D  
> **username_1**: This was a comment!! You seem to need more guidance, ask your advisor or a senior people how to find the most expressed genes and from there how to find it those that are so in several stages.  

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