<p>Usually it is more problematic to work with older versions of data with newer protocols than the other way around.</p>

<p>In this case, I think that the features annotated in the (newer) bed file should be more accurate than the older one. Unless you specifically want to compare your experiment with a previous experiment performed with a bed file from 2012 you'll be fine using the newer one. </p>

<p>However make sure that you are correctly using the right file and that you report (or record) each version of protocol and data used in case some doubts arise. </p>

CC BY-SA 3.0
2017-11-17T07:55:28.080Z
2017-11-17T15:37:00.930Z
2017-11-17T15:37:00.930Z
llrs
<p>I have to run an analysis using Whole Exome Sequencing (WXS) data prepared with the TruSeq Exome Kit (2012).</p>

<p>I need the <code>.bed</code> file for the kit and I found it <a href="https://support.illumina.com/sequencing/sequencing_kits/truseq-exome-kit/downloads.html" rel="nofollow noreferrer">here</a>.</p>

<p>However, this file was posted on Nov. 16, 2015, while the kit used in my case is from 2012. I searched but I didn't find the bedfile for that specific version online.</p>

<p>Will I run into problems if I use this version, and in this case, where can I retrieve the bedfile for 2012's version?</p>

CC BY-SA 3.0
2017-11-17T15:29:14.810Z
Care to explain why the downvote?
gc5
CC BY-SA 3.0
2017-11-17T15:36:43.977Z
I haven't down voted you, but you don't show what have you done to understand what problems can you have when using an older file, nor the places you have searched for the bed files of 2012. Or maybe it was from biology.se, who knows ?
llrs
CC BY-SA 3.0
2017-11-17T15:44:11.900Z
I usually search before writing a question, so also in this case I searched online to understand what could be the difference. Since online I couldn't find anything I wrote this question. Also, the fact that I put the reference to the Illumina kit page (the only page in which I could find reference to the bedfile of the kit) disproves that I didn't put the places I have searched. So I don't see the point of downvoting, since I: clearly stated the problem, provided details about what I've done, provided links to what I've found.
gc5
CC BY-SA 3.0
2017-11-17T16:04:04.980Z
Well, those are imaginary internet points, don't mind them too much!
llrs
CC BY-SA 3.0
2017-11-17T16:15:18.187Z
Actually I don't mind them :) but since I put some effort to search and write I couldn't understand why the downvote.
gc5
<bed><exome>
TruSeq Exome Kit bed file old version
# TruSeq Exome Kit bed file old version

(Asked by: username_0 on 2017-10-11)

I have to run an analysis using Whole Exome Sequencing (WXS) data prepared with the TruSeq Exome Kit (2012).

I need the `.bed` file for the kit and I found it [here](https://support.illumina.com/sequencing/sequencing_kits/truseq-exome-kit/downloads.html).

However, this file was posted on Nov. 16, 2015, while the kit used in my case is from 2012. I searched but I didn't find the bedfile for that specific version online.

Will I run into problems if I use this version, and in this case, where can I retrieve the bedfile for 2012's version?

**Tags:** bed, exome
> **Comments:**
> **username_0**: Care to explain why the downvote?  
> **username_1**: I haven't down voted you, but you don't show what have you done to understand what problems can you have when using an older file, nor the places you have searched for the bed files of 2012. Or maybe it was from biology.se, who knows ?  
> **username_0**: I usually search before writing a question, so also in this case I searched online to understand what could be the difference. Since online I couldn't find anything I wrote this question. Also, the fact that I put the reference to the Illumina kit page (the only page in which I could find reference to the bedfile of the kit) disproves that I didn't put the places I have searched. So I don't see the point of downvoting, since I: clearly stated the problem, provided details about what I've done, provided links to what I've found.  
> **username_1**: Well, those are imaginary internet points, don't mind them too much!  
> **username_0**: Actually I don't mind them :) but since I put some effort to search and write I couldn't understand why the downvote.  

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## 1 answers

### Answer by username_1 on 2017-11-17. Score: 0 | Accepted answer

Usually it is more problematic to work with older versions of data with newer protocols than the other way around.

In this case, I think that the features annotated in the (newer) bed file should be more accurate than the older one. Unless you specifically want to compare your experiment with a previous experiment performed with a bed file from 2012 you'll be fine using the newer one.

However make sure that you are correctly using the right file and that you report (or record) each version of protocol and data used in case some doubts arise.


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