<p>It is possible, with tools like <a href="https://crossmap.readthedocs.io/en/latest/" rel="nofollow noreferrer">CrossMap</a>, but my recommendation is to re-align. Assemblies can change a lot between versions and your reads will undoubdedly align differently. Re-aligning is cheap, it will take a few days and you're good. Re-doing your analysis because you realize the coordinate translation messed something up will cost you much more time and energy.</p>

CC BY-SA 4.0
2020-11-13T22:12:31.050Z
2020-11-13T22:12:31.050Z
Bastian Schiffthaler
<p>I Have multiple BAM files that are referenced to UCSC genome assembly GRCh37/hg19 that are read in different time frames. Now, I am planning a different studies that require assembling all the data against one assembly. Will it be possible to convert all the BAM files that I have to an upgraded version or convert the assembly to a different new assembly and how?
I am new in this and not a bioinformatician so, if you have any questions regarding my question to answer you can ask, any type of help is highly appreciated.</p>

<sam><genome><assembly>
Is it possible to convert BAM file from one genome assembly to the other?
# Is it possible to convert BAM file from one genome assembly to the other?

(Asked by: username_0 on 2020-11-13)

I Have multiple BAM files that are referenced to UCSC genome assembly GRCh37/hg19 that are read in different time frames. Now, I am planning a different studies that require assembling all the data against one assembly. Will it be possible to convert all the BAM files that I have to an upgraded version or convert the assembly to a different new assembly and how?
I am new in this and not a bioinformatician so, if you have any questions regarding my question to answer you can ask, any type of help is highly appreciated.

**Tags:** sam, genome, assembly

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## 1 answers

### Answer by username_1 on 2020-11-13. Score: 1

It is possible, with tools like [CrossMap](https://crossmap.readthedocs.io/en/latest/), but my recommendation is to re-align. Assemblies can change a lot between versions and your reads will undoubdedly align differently. Re-aligning is cheap, it will take a few days and you're good. Re-doing your analysis because you realize the coordinate translation messed something up will cost you much more time and energy.


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