<p>I'd like to ask you for help with an university assignment. First let me post the entire question:</p>
<blockquote>
<p>Design primers for bisulfite sequencing of the promoter of the ACE2
gene in microbat  Myotis lucifugus (based on the annotation of the
Broad Institute Myoluc2.0/myoLuc2). The gene is localized on the
contig GL429816. As a transcription start, use the start of the ACE2
transcript variant XM_023753670.1; once you find ACE2 in the UCSC
genome browser .You will find it in NCBI RefSeq track</p>
</blockquote>
<p>I don't want anyone to solve it for me but I do have a problem with one aspect. I am not clear on how to determine what is a valid promoter for this gene in microbat. I do know it is the upstream sequence however I am not clear on how to tell the number of upstream bases that are the actual promoter. I did find some sources for the actual promoter sequence determined by much smarter people than myself however those are for ACE in humans so I am afraid that it is not applicable in my case.</p>
<p>I'd like someone to point me in the right direction where I could find my answer would be enough.</p>

CC BY-SA 4.0
2021-02-13T17:49:40.470Z
Thank you I decided to use 900bp, just because that is the legnth the teacher used in of his lectures. I would like to select you comment as an answer, but it's not possible it seems.
Matěj Nemec
<gene><ngs><methylation><homework><epigenetics>
How to determine a correct promoter sequence? (Bisulfite sequencing)
# How to determine a correct promoter sequence? (Bisulfite sequencing)

(Asked by: username_0 on 2021-02-12)

I'd like to ask you for help with an university assignment. First let me post the entire question:

> Design primers for bisulfite sequencing of the promoter of the ACE2
> gene in microbat Myotis lucifugus (based on the annotation of the
> Broad Institute Myoluc2.0/myoLuc2). The gene is localized on the
> contig GL429816. As a transcription start, use the start of the ACE2
> transcript variant XM\_023753670.1; once you find ACE2 in the UCSC
> genome browser .You will find it in NCBI RefSeq track

I don't want anyone to solve it for me but I do have a problem with one aspect. I am not clear on how to determine what is a valid promoter for this gene in microbat. I do know it is the upstream sequence however I am not clear on how to tell the number of upstream bases that are the actual promoter. I did find some sources for the actual promoter sequence determined by much smarter people than myself however those are for ACE in humans so I am afraid that it is not applicable in my case.

I'd like someone to point me in the right direction where I could find my answer would be enough.

**Tags:** gene, ngs, methylation, homework, epigenetics
> **Comments:**
> **username_0**: Thank you I decided to use 900bp, just because that is the legnth the teacher used in of his lectures. I would like to select you comment as an answer, but it's not possible it seems.  

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## 0 answers

