<p>It is my first time using Chimera. I have uploaded a PDB file on to Chimera and opened it up on the software. I need to change the threshold of the EM map so that I can analyze what the EM map would look like with a threshold too high and too low.</p>
<p>Please let me know if you have any suggestions.</p>
<p>Thanks</p>

CC BY-SA 4.0
2022-02-11T14:22:25.007Z
Could you supply more details on the goal of your analysis?
M__
CC BY-SA 4.0
2022-02-11T14:22:39.363Z
https://www.cgl.ucsf.edu/chimera/current/docs/ContributedSoftware/volumeviewer/framevolumeviewer.html
pippo1980
CC BY-SA 4.0
2022-02-11T14:32:18.127Z
I am trying to view a protein with a threshold that Is too high, a threshold that is too low, and finally, the correct threshold to se the secondary structures.
microbeprincess
<proteins><protein-structure>
Chimera- How to go about changing the threshold of the EM map?
# Chimera- How to go about changing the threshold of the EM map?

(Asked by: username_0 on 2022-02-11)

It is my first time using Chimera. I have uploaded a PDB file on to Chimera and opened it up on the software. I need to change the threshold of the EM map so that I can analyze what the EM map would look like with a threshold too high and too low.

Please let me know if you have any suggestions.

Thanks

**Tags:** proteins, protein-structure
> **Comments:**
> **username_1**: Could you supply more details on the goal of your analysis?  
> **username_2**: https://www.cgl.ucsf.edu/chimera/current/docs/ContributedSoftware/volumeviewer/framevolumeviewer.html  
> **username_0**: I am trying to view a protein with a threshold that Is too high, a threshold that is too low, and finally, the correct threshold to se the secondary structures.  

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## 0 answers

