<p>PacBio lima need both 5'end and 3' end barcodes. You can have a unique barcode in one end and multiple on the other end; but it need two barcodes.</p>

CC BY-SA 4.0
2024-01-03T01:04:31.737Z
2024-01-03T01:04:31.737Z
user3377241
<p>I was wondering if there is a way to provide pacbio lima demultiplexing only a barcode, and let  lima demux sample even if only one end has the barcode, not both?</p>

CC BY-SA 4.0
2023-12-21T09:08:36.750Z
Can you please explain more about the context of your problem? What are you trying to do with the demultiplexing; what is the ultimate aim of the analysis?
gringer
CC BY-SA 4.0
2023-12-21T19:00:06.197Z
Some examples of what your data look like might help
Maximilian Press
<pacbio><demultiplexing>
pacbio demultiplexing issue
# pacbio demultiplexing issue

(Asked by: username_0 on 2023-12-19)

I was wondering if there is a way to provide pacbio lima demultiplexing only a barcode, and let lima demux sample even if only one end has the barcode, not both?

**Tags:** pacbio, demultiplexing
> **Comments:**
> **username_1**: Can you please explain more about the context of your problem? What are you trying to do with the demultiplexing; what is the ultimate aim of the analysis?  
> **username_2**: Some examples of what your data look like might help  

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## 1 answers

### Answer by username_3 on 2024-01-03. Score: -1

PacBio lima need both 5'end and 3' end barcodes. You can have a unique barcode in one end and multiple on the other end; but it need two barcodes.


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