<p>How do you verify if a RNAseq dataset is unstranded, stranded or revesely stranded from a fastq file?</p>

CC BY-SA 4.0
2021-01-27T09:15:15.067Z
I was looking for a method that didn't involve quasi-mapping but I think this will solve my problem. Thank you!
indigoblueraspberry
CC BY-SA 4.0
2021-01-27T09:18:09.283Z
Unless the metadata is recorded somewhere you won’t get around mapping: the FASTQs themselves don’t record this information anywhere.
Konrad Rudolph
<rna-seq><fastq>
How do you determine the strandedness of a RNAseq dataset?
# How do you determine the strandedness of a RNAseq dataset?

(Asked by: username_0 on 2021-01-26)

How do you verify if a RNAseq dataset is unstranded, stranded or revesely stranded from a fastq file?

**Tags:** rna-seq, fastq
> **Comments:**
> **username_0**: I was looking for a method that didn't involve quasi-mapping but I think this will solve my problem. Thank you!  
> **username_1**: Unless the metadata is recorded somewhere you won’t get around mapping: the FASTQs themselves don’t record this information anywhere.  

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## 0 answers

