<p>"Feature Barcoding" refers to using antibodies linked to specific oligos. The antibodies can then bind to cell surface markers of interest and the oligos they're conjugated with turned into normal scRNA-seq reads. This effectively means you can get a FACs profile for each cell without needing to run it through a FACs sorter and individually label it.</p>

<p>The "feature barcoding" nomenclature comes from 10X I think, but there are a few similar protocols (maybe they're exactly the same in the end, I'm not sure about the details) such as <a href="https://www.nature.com/articles/nmeth.4380" rel="nofollow noreferrer">CITE-seq</a> and <a href="https://www.nature.com/articles/nbt.3973" rel="nofollow noreferrer">REAP-seq</a>. You can see a talk from 10X on this <a href="https://www.youtube.com/watch?v=jzVMVAO7Af0" rel="nofollow noreferrer">here</a>.</p>

CC BY-SA 4.0
2019-02-10T14:10:51.653Z
Thank you so much. Now it's more clear for me. May you suggest any paper come with it? I found some paper but don't know which one is better for understanding: `https://www.nature.com/articles/nmeth.4380` or `https://www.biorxiv.org/content/10.1101/237693v1`
Trong-Thang Pham
CC BY-SA 4.0
2019-02-10T19:53:04.267Z
I've updated my answer, the nature methods paper you linked to at least is related (I mentioned it in my update). The cell hashing paper you mentioned is also very related.
Devon Ryan
CC BY-SA 4.0
2019-02-11T02:03:15.310Z
Thanks. I think this is it. I didn't know there is a talk about it. Thank you again for your answer. I will dig into it.
Trong-Thang Pham
CC BY-SA 4.0
2019-02-10T12:12:19.620Z
2019-02-10T19:50:52.333Z
2019-02-10T19:50:52.333Z
Devon Ryan
<p>I heard the term Feature Barcoding a lot so I find resources to understand it. I have read docs of 10xgenomics and I see that <code>Feature Barcoding technology is a method for adding extra channels of information to cells by running single-cell gene expression in parallel with other assays</code>, this technology also do something with t-SNE and something with matrices. </p>

<p>I can't imagine the whole picture because I don't know what are those <code>adding extra channels of information to cells</code>, <code>single-cell gene expression</code> and <code>parallel with other assays</code> mean. And what is the input and output of this technology? Is there any paper go along with this technology?</p>

CC BY-SA 4.0
2019-02-10T03:40:27.137Z
Welcome to the site.  Have you looked at the 10X genomics website?  Usually they post all the papers and materials.  How you done pubmed or google scholar search for papers?
Bioathlete
CC BY-SA 4.0
2019-02-10T14:18:54.477Z
I did but I only found this paper relates to the term "Feature Barcoding" when some people tweeted about `https://www.biorxiv.org/content/10.1101/237693v1` , and this paper in Feature Barcoding Glossary `https://www.nature.com/articles/nmeth.4380` . But I cannot find the whole match "Feature Barcoding" in that paper which make me unclear how it relates to each other.
Trong-Thang Pham
<barcode><features>
What is Feature Barcoding technology?
# What is Feature Barcoding technology?

(Asked by: username_0 on 2019-02-10)

I heard the term Feature Barcoding a lot so I find resources to understand it. I have read docs of 10xgenomics and I see that `Feature Barcoding technology is a method for adding extra channels of information to cells by running single-cell gene expression in parallel with other assays`, this technology also do something with t-SNE and something with matrices.

I can't imagine the whole picture because I don't know what are those `adding extra channels of information to cells`, `single-cell gene expression` and `parallel with other assays` mean. And what is the input and output of this technology? Is there any paper go along with this technology?

**Tags:** barcode, features
> **Comments:**
> **username_1**: Welcome to the site.  Have you looked at the 10X genomics website?  Usually they post all the papers and materials.  How you done pubmed or google scholar search for papers?  
> **username_0**: I did but I only found this paper relates to the term "Feature Barcoding" when some people tweeted about `https://www.biorxiv.org/content/10.1101/237693v1` , and this paper in Feature Barcoding Glossary `https://www.nature.com/articles/nmeth.4380` . But I cannot find the whole match "Feature Barcoding" in that paper which make me unclear how it relates to each other.  

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## 1 answers

### Answer by username_2 on 2019-02-10. Score: 3 | Accepted answer

"Feature Barcoding" refers to using antibodies linked to specific oligos. The antibodies can then bind to cell surface markers of interest and the oligos they're conjugated with turned into normal scRNA-seq reads. This effectively means you can get a FACs profile for each cell without needing to run it through a FACs sorter and individually label it.

The "feature barcoding" nomenclature comes from 10X I think, but there are a few similar protocols (maybe they're exactly the same in the end, I'm not sure about the details) such as [CITE-seq](https://www.nature.com/articles/nmeth.4380) and [REAP-seq](https://www.nature.com/articles/nbt.3973). You can see a talk from 10X on this [here](https://www.youtube.com/watch?v=jzVMVAO7Af0).

> **Comments:**
> **username_0**: Thank you so much. Now it's more clear for me. May you suggest any paper come with it? I found some paper but don't know which one is better for understanding: `https://www.nature.com/articles/nmeth.4380` or `https://www.biorxiv.org/content/10.1101/237693v1`  
> **username_2**: I've updated my answer, the nature methods paper you linked to at least is related (I mentioned it in my update). The cell hashing paper you mentioned is also very related.  
> **username_0**: Thanks. I think this is it. I didn't know there is a talk about it. Thank you again for your answer. I will dig into it.  

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