<p>Currently, there is no available mitochondrial sequence for the ROS_Cfam_1.0 assembly:
<a href="https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_014441545.1/" rel="nofollow noreferrer">https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_014441545.1/</a></p>

CC BY-SA 4.0
2024-01-18T15:01:50.280Z
Thank you for your reply Ben!
Alexandria Kouri
CC BY-SA 4.0
2024-01-18T14:56:27.050Z
2024-01-18T14:56:27.050Z
Ben_Moore
<p>I have an RNA-Seq and scRNA-Seq dataset from dog samples and pseudo-aligned them to the dog reference transcriptome (Ensembl release 109). When filtering for mitochondrial gene percentage in scRNA-Seq I couldn't find the &quot;MT&quot; annotation in this new release. Does anyone have any information about this? Did they not include it or am I doing something wrong somewhere (the MT annotations are present in the CanFam3.1 genome built but not in the newer ones).
I could not find anything on the Ensembl release details or blog.</p>
<p>Thank you :)</p>
<p>This is a part of the code that I used to search for the mitochondrial genes:</p>
<pre><code>searchFilterOptions(mart=ensembl_canis, filter=&quot;chromosome_name&quot;, pattern=&quot;MT&quot;)
</code></pre>

<sequence-annotation><ensembl>
Dog (Canis lupus familiaris) MT gene annotations
# Dog (Canis lupus familiaris) MT gene annotations

(Asked by: username_0 on 2024-01-18)

I have an RNA-Seq and scRNA-Seq dataset from dog samples and pseudo-aligned them to the dog reference transcriptome (Ensembl release 109). When filtering for mitochondrial gene percentage in scRNA-Seq I couldn't find the "MT" annotation in this new release. Does anyone have any information about this? Did they not include it or am I doing something wrong somewhere (the MT annotations are present in the CanFam3.1 genome built but not in the newer ones).
I could not find anything on the Ensembl release details or blog.
Thank you :)
This is a part of the code that I used to search for the mitochondrial genes:
searchFilterOptions(mart=ensembl_canis, filter="chromosome_name", pattern="MT")

**Tags:** sequence-annotation, ensembl

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## 1 answers

### Answer by username_1 on 2024-01-18. Score: 3 | Accepted answer

Currently, there is no available mitochondrial sequence for the ROS\_Cfam\_1.0 assembly:
<https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_014441545.1/>

> **Comments:**
> **username_0**: Thank you for your reply Ben!  

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