<p>Mates in a pair are giving signal for a single strand, though this is only apparent in bisulfite-treated samples. The reason for this is that you are sequencing the ends of fragments of material and it's random which strand of this (or both in different clusters) will get sequenced. Different pairs of reads will then randomly sequence one strand or the other.</p>

<p>Single-end reads are the same as paired-end reads, the second read in the pair is just not made.</p>

CC BY-SA 4.0
2020-03-26T15:08:17.073Z
Now, I understood from you that the mates in paired-end reads are coming from two different strands, but based on the figure in https://www.biostars.org/p/95803/ and also some other sources, I implied that they are coming from two different stands as they are read from two different directions. Could you please help me in understanding this?
Negin
CC BY-SA 4.0
2020-03-25T21:55:57.633Z
2020-03-25T21:55:57.633Z
Devon Ryan
<p>I have questions regarding sequencing:</p>

<p>Are paired-end reads from different stands?
What about single reads, are they coming from both strands?</p>

<phylogenetics><ngs><genome><sequence-analysis><genome-sequencing>
Location of Reads in Sequencing
# Location of Reads in Sequencing

(Asked by: username_0 on 2020-03-25)

I have questions regarding sequencing:

Are paired-end reads from different stands?
What about single reads, are they coming from both strands?

**Tags:** phylogenetics, ngs, genome, sequence-analysis, genome-sequencing

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## 1 answers

### Answer by username_1 on 2020-03-25. Score: 2

Mates in a pair are giving signal for a single strand, though this is only apparent in bisulfite-treated samples. The reason for this is that you are sequencing the ends of fragments of material and it's random which strand of this (or both in different clusters) will get sequenced. Different pairs of reads will then randomly sequence one strand or the other.

Single-end reads are the same as paired-end reads, the second read in the pair is just not made.

> **Comments:**
> **username_0**: Now, I understood from you that the mates in paired-end reads are coming from two different strands, but based on the figure in https://www.biostars.org/p/95803/ and also some other sources, I implied that they are coming from two different stands as they are read from two different directions. Could you please help me in understanding this?  

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