<p>Yes, just add up the coverage at each location; that's integration in a nutshell.</p>

<p>Or, alternatively / equivalently, count the total number of reads within the region.</p>

CC BY-SA 4.0
2018-10-16T21:16:25.643Z
2018-10-16T21:16:25.643Z
gringer
<p>Is there a way to calculate the area under a curve in a coverage graph?</p>

<p>Thank you in advance.</p>

<p>EDIT:</p>

<p>I'd like to calculate the AUC per strand per gene in a coverage graph. I have two wig files (sense and antisense) generated from a single bam (RNA-seq) file (using the <a href="https://github.com/pmenzel/stranded-coverage" rel="nofollow noreferrer">strand-coverage</a> software).</p>

CC BY-SA 4.0
2018-10-17T14:16:37.643Z
Welcome to the group.  Can you expand on your question to include what tools you are using, what you have tried.  How did you generate the curve and do you have any sample data to help answer your question?
Bioathlete
CC BY-SA 4.0
2018-10-17T15:22:19.187Z
Hi Bioathlete. I'm totally new in Bioinformatics field. I have not tried much yet due to lack of knowledge, but I edited my question. Thank you.
Guzto
<rna-seq><coverage>
How to calculate AUC in coverage graph
# How to calculate AUC in coverage graph

(Asked by: username_0 on 2018-10-16)

Is there a way to calculate the area under a curve in a coverage graph?

Thank you in advance.

EDIT:

I'd like to calculate the AUC per strand per gene in a coverage graph. I have two wig files (sense and antisense) generated from a single bam (RNA-seq) file (using the [strand-coverage](https://github.com/pmenzel/stranded-coverage) software).

**Tags:** rna-seq, coverage
> **Comments:**
> **username_1**: Welcome to the group.  Can you expand on your question to include what tools you are using, what you have tried.  How did you generate the curve and do you have any sample data to help answer your question?  
> **username_0**: Hi Bioathlete. I'm totally new in Bioinformatics field. I have not tried much yet due to lack of knowledge, but I edited my question. Thank you.  

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## 1 answers

### Answer by username_2 on 2018-10-16. Score: 4 | Accepted answer

Yes, just add up the coverage at each location; that's integration in a nutshell.

Or, alternatively / equivalently, count the total number of reads within the region.


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