<p>There are several programs for simulating/predicting DNA melting but you could try them for RNA.
You can look here <a href="http://bioinformatics.org/meltsim/wiki/Main/HomePage" rel="nofollow noreferrer">http://bioinformatics.org/meltsim/wiki/Main/HomePage</a>.</p>

CC BY-SA 4.0
2020-03-03T22:47:57.333Z
2020-03-03T22:47:57.333Z
02213 The Godfather
<p>Both <a href="https://biopython.org/docs/1.75/api/Bio.SeqUtils.MeltingTemp.html" rel="nofollow noreferrer">Bio.SeqUtils.MeltingTemp</a> of Biopython and <a href="https://primer3.org/" rel="nofollow noreferrer">Primer3</a> offers different methods/algorithms to calculate the Tm value.</p>

CC BY-SA 4.0
2021-07-27T10:44:00.697Z
2021-07-27T10:44:00.697Z
haci
<p>Is there any tool available to predict the melting temperature for rna/lna oligos with its perfect complement ? </p>

<p>I know this amazing website from qiagen/geneglobe that estimates the tm value. </p>

<p>But is there any command line tool that does this? </p>

<p>I can estimate the normal tm value with RNAcofold and the -T option but there is no lna option. </p>

<p>I searched for a while but i can only find online services. </p>

<rna>
Tool for rna/lna melting temperature prediction
# Tool for rna/lna melting temperature prediction

(Asked by: username_0 on 2020-03-03)

Is there any tool available to predict the melting temperature for rna/lna oligos with its perfect complement ?

I know this amazing website from qiagen/geneglobe that estimates the tm value.

But is there any command line tool that does this?

I can estimate the normal tm value with RNAcofold and the -T option but there is no lna option.

I searched for a while but i can only find online services.

**Tags:** rna

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## 2 answers

### Answer by username_1 on 2020-03-03. Score: 1

There are several programs for simulating/predicting DNA melting but you could try them for RNA.
You can look here <http://bioinformatics.org/meltsim/wiki/Main/HomePage>.


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### Answer by username_2 on 2021-07-27. Score: 1

Both [Bio.SeqUtils.MeltingTemp](https://biopython.org/docs/1.75/api/Bio.SeqUtils.MeltingTemp.html) of Biopython and [Primer3](https://primer3.org/) offers different methods/algorithms to calculate the Tm value.


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