<p>I am doing an RNAseq experiment with affected (n=6) and control groups (n=6) in cow. However it turned out that 4 of my control samples have very low quality so I have to discard them.
My problem is that there is no way to repeat the sampling and sequencing.
Are there any solutions to perform DE,GSEA with these conditions (somehow generating bootstrapped samples from control groups or something similar)?
Thanks in advance!</p>

<rna-seq><differential-expression><gsea>
RNAseq small sample size in control group
# RNAseq small sample size in control group

(Asked by: username_0 on 2022-03-31)

I am doing an RNAseq experiment with affected (n=6) and control groups (n=6) in cow. However it turned out that 4 of my control samples have very low quality so I have to discard them.
My problem is that there is no way to repeat the sampling and sequencing.
Are there any solutions to perform DE,GSEA with these conditions (somehow generating bootstrapped samples from control groups or something similar)?
Thanks in advance!

**Tags:** rna-seq, differential-expression, gsea

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## 0 answers

