<p>You can use <a href="https://www.ebi.ac.uk/Tools/seqstats/emboss_pepstats/" rel="nofollow noreferrer">EMBOSS Pepstats</a> for this. It takes a multi-fasta as input and produces a table that contains various statistics related to the protein, including isoelectric point.</p>

CC BY-SA 4.0
2020-07-13T11:14:08.837Z
2020-07-13T11:14:08.837Z
vkkodali
<p>I need to calculate Isoelectric point from a multifasta file, is there any python code or web tool that allows me to do that?</p>

CC BY-SA 4.0
2020-07-13T09:59:39.027Z
Can you use Python (biopython has a very straightforward method for pI calculations, `Bio.SeqUtils.IsoelectricPoint`)?
Matteo Ferla
<fasta><proteins>
Calculating Isoelectric point from a multifasta file
# Calculating Isoelectric point from a multifasta file

(Asked by: username_0 on 2020-07-13)

I need to calculate Isoelectric point from a multifasta file, is there any python code or web tool that allows me to do that?

**Tags:** fasta, proteins
> **Comments:**
> **username_1**: Can you use Python (biopython has a very straightforward method for pI calculations, `Bio.SeqUtils.IsoelectricPoint`)?  

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## 1 answers

### Answer by username_2 on 2020-07-13. Score: 4

You can use [EMBOSS Pepstats](https://www.ebi.ac.uk/Tools/seqstats/emboss_pepstats/) for this. It takes a multi-fasta as input and produces a table that contains various statistics related to the protein, including isoelectric point.


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