<p>I have done a study on isoform switch between different tissue type. Now, many people asking me to study alternative splicing also. But, dont they will give me same results? If there is a significant splicing difference; that will definitely leads to isoform switch. Or my understanding is incorrect ?</p>

<p>Thanks</p>

CC BY-SA 4.0
2020-05-23T22:02:05.817Z
Imaging isoform A uses exon 1, and isoform B uses exon 2. Now if you do an isoform switch analysis, you might see that in one condition isoform A (and exon 1) is preferred over isoform B (and exon 2). You might get similar results for alternative splicing. But if there is another isoform C that uses both exon 1 and 2, then the alternative splicing analysis of these exons are going to be affected by the expression level of isoform C as well.
Phoenix Mu
CC BY-SA 4.0
2020-06-07T02:55:23.863Z
Thanks @PhoenixMu
user3377241
<differential-expression><rna-splicing><isoform>
Do I need to study alternative splicing and isoformswitch separately?
# Do I need to study alternative splicing and isoformswitch separately?

(Asked by: username_0 on 2020-05-23)

I have done a study on isoform switch between different tissue type. Now, many people asking me to study alternative splicing also. But, dont they will give me same results? If there is a significant splicing difference; that will definitely leads to isoform switch. Or my understanding is incorrect ?

Thanks

**Tags:** differential-expression, rna-splicing, isoform
> **Comments:**
> **username_1**: Imaging isoform A uses exon 1, and isoform B uses exon 2. Now if you do an isoform switch analysis, you might see that in one condition isoform A (and exon 1) is preferred over isoform B (and exon 2). You might get similar results for alternative splicing. But if there is another isoform C that uses both exon 1 and 2, then the alternative splicing analysis of these exons are going to be affected by the expression level of isoform C as well.  
> **username_0**: Thanks @PhoenixMu  

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## 0 answers

