<p>Sequencing also requires single stranded DNA, otherwise the sequencing primers can’t bind. Sure there are steps in the protocol where double stranded material exists, but that’s transient. </p>

CC BY-SA 4.0
2018-05-20T06:47:44.827Z
2018-05-20T06:47:44.827Z
Devon Ryan
<p>Single stranded cDNA is used by race pcr and rt-pcr while next-gen sequencing (Illumina) requires double stranded cDNA. What is the reason behind this ? </p>

CC BY-SA 4.0
2018-05-22T13:27:04.923Z
I'm voting to close this question as off-topic because the question is not about bioinformatics, but for some specific methods of the wet lab. If you have a question about how to handle these sequences by computers, I think it would be better suited for the site.
llrs
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2018-05-22T14:41:19.513Z
Can we migrate this to biology? It's a valid question and I think it's even somewhat relevant to bioinformatic (it's important to understand generation of the data), but it would suit better to Biology.SE I think. Anyway, could you link source of your information?
Kamil S Jaron
<phylogenetics>
single stranded cDNA vs double-stranded cDNA
# single stranded cDNA vs double-stranded cDNA

(Asked by: username_0 on 2018-05-19)

Single stranded cDNA is used by race pcr and rt-pcr while next-gen sequencing (Illumina) requires double stranded cDNA. What is the reason behind this ?

**Tags:** phylogenetics
> **Comments:**
> **username_1**: I'm voting to close this question as off-topic because the question is not about bioinformatics, but for some specific methods of the wet lab. If you have a question about how to handle these sequences by computers, I think it would be better suited for the site.  
> **username_2**: Can we migrate this to biology? It's a valid question and I think it's even somewhat relevant to bioinformatic (it's important to understand generation of the data), but it would suit better to Biology.SE I think. Anyway, could you link source of your information?  

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## 1 answers

### Answer by username_3 on 2018-05-20. Score: 1 | Accepted answer

Sequencing also requires single stranded DNA, otherwise the sequencing primers can’t bind. Sure there are steps in the protocol where double stranded material exists, but that’s transient.


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