Expression of ENaC in LPS-induced inflammation of middle ear mucosa
Jae-Jun Song, Seong Keun Kwon, Chang Gun Cho, Seok Won Park, Sungwon Chae
Department of Otorhinolaryngology-Head and Neck Surgery
Conclusion: The expression of all three subunits of the epithelial sodium channel (ENaC) decreased after the lipopolysaccharide (LPS) injection and normalized as the fluid collection resolved. This implies that fluid collection in acute otitis media may be caused by the inhibition of ENaC function. Objectives: We investigated the expression levels of subunits of ENaC after an injection of LPS into the middle ear cavity of rats. Methods: Seventy-two Sprague-Dawley rats were assigned randomly into the five groups that received an LPS injection and the control group. A transtympanic injection of LPS (1 mg/ml) was done and rats were sacrificed 6, 12, 24, 72, and 120 h after the procedure. Real-time RT-PCR was carried out for ENaC-α, -β, -γ, and immunohistochemistry was performed for ENaC-α and -β. Results: The level of ENaC-α expression decreased 0.50-fold and 0.55-fold at 6 and 12 h, respectively (p < 0.05), but it normalized at 24 h. It increased 3.64-fold at 72 h and 3.24-fold at 120 h (p < 0.05). At 6 and 12 h after the LPS injection into the middle ear cavity, inflammation was induced and ENaC-α immunoreactivity decreased. At 24 h, ENaC-α immunoreactivity was normalized, then at 72 and 120 h after the injection it was increased.
Acta Oto-Laryngologica
Published - 2012 Nov 1
Epithelial sodium channel
Inflammatory response
Song, J-J., Kwon, S. K., Cho, C. G., Park, S. W., & Chae, S. (2012). Expression of ENaC in LPS-induced inflammation of middle ear mucosa. Acta Oto-Laryngologica, 132(11), 1145-1150. https://doi.org/10.3109/00016489.2012.697640
Expression of ENaC in LPS-induced inflammation of middle ear mucosa. / Song, Jae-Jun; Kwon, Seong Keun; Cho, Chang Gun; Park, Seok Won; Chae, Sungwon.
In: Acta Oto-Laryngologica, Vol. 132, No. 11, 01.11.2012, p. 1145-1150.
Song, J-J, Kwon, SK, Cho, CG, Park, SW & Chae, S 2012, 'Expression of ENaC in LPS-induced inflammation of middle ear mucosa', Acta Oto-Laryngologica, vol. 132, no. 11, pp. 1145-1150. https://doi.org/10.3109/00016489.2012.697640
Song J-J, Kwon SK, Cho CG, Park SW, Chae S. Expression of ENaC in LPS-induced inflammation of middle ear mucosa. Acta Oto-Laryngologica. 2012 Nov 1;132(11):1145-1150. https://doi.org/10.3109/00016489.2012.697640
Song, Jae-Jun ; Kwon, Seong Keun ; Cho, Chang Gun ; Park, Seok Won ; Chae, Sungwon. / Expression of ENaC in LPS-induced inflammation of middle ear mucosa. In: Acta Oto-Laryngologica. 2012 ; Vol. 132, No. 11. pp. 1145-1150.
@article{39ca5c86405a4fa1b273cb3b1575fd44,
title = "Expression of ENaC in LPS-induced inflammation of middle ear mucosa",
abstract = "Conclusion: The expression of all three subunits of the epithelial sodium channel (ENaC) decreased after the lipopolysaccharide (LPS) injection and normalized as the fluid collection resolved. This implies that fluid collection in acute otitis media may be caused by the inhibition of ENaC function. Objectives: We investigated the expression levels of subunits of ENaC after an injection of LPS into the middle ear cavity of rats. Methods: Seventy-two Sprague-Dawley rats were assigned randomly into the five groups that received an LPS injection and the control group. A transtympanic injection of LPS (1 mg/ml) was done and rats were sacrificed 6, 12, 24, 72, and 120 h after the procedure. Real-time RT-PCR was carried out for ENaC-α, -β, -γ, and immunohistochemistry was performed for ENaC-α and -β. Results: The level of ENaC-α expression decreased 0.50-fold and 0.55-fold at 6 and 12 h, respectively (p < 0.05), but it normalized at 24 h. It increased 3.64-fold at 72 h and 3.24-fold at 120 h (p < 0.05). At 6 and 12 h after the LPS injection into the middle ear cavity, inflammation was induced and ENaC-α immunoreactivity decreased. At 24 h, ENaC-α immunoreactivity was normalized, then at 72 and 120 h after the injection it was increased.",
keywords = "Epithelial sodium channel, Inflammatory response, Lipopolysaccharide, Otitis media",
author = "Jae-Jun Song and Kwon, {Seong Keun} and Cho, {Chang Gun} and Park, {Seok Won} and Sungwon Chae",
journal = "Acta Oto-Laryngologica",
publisher = "Informa Healthcare",
T1 - Expression of ENaC in LPS-induced inflammation of middle ear mucosa
AU - Song, Jae-Jun
AU - Kwon, Seong Keun
AU - Cho, Chang Gun
AU - Park, Seok Won
AU - Chae, Sungwon
N2 - Conclusion: The expression of all three subunits of the epithelial sodium channel (ENaC) decreased after the lipopolysaccharide (LPS) injection and normalized as the fluid collection resolved. This implies that fluid collection in acute otitis media may be caused by the inhibition of ENaC function. Objectives: We investigated the expression levels of subunits of ENaC after an injection of LPS into the middle ear cavity of rats. Methods: Seventy-two Sprague-Dawley rats were assigned randomly into the five groups that received an LPS injection and the control group. A transtympanic injection of LPS (1 mg/ml) was done and rats were sacrificed 6, 12, 24, 72, and 120 h after the procedure. Real-time RT-PCR was carried out for ENaC-α, -β, -γ, and immunohistochemistry was performed for ENaC-α and -β. Results: The level of ENaC-α expression decreased 0.50-fold and 0.55-fold at 6 and 12 h, respectively (p < 0.05), but it normalized at 24 h. It increased 3.64-fold at 72 h and 3.24-fold at 120 h (p < 0.05). At 6 and 12 h after the LPS injection into the middle ear cavity, inflammation was induced and ENaC-α immunoreactivity decreased. At 24 h, ENaC-α immunoreactivity was normalized, then at 72 and 120 h after the injection it was increased.
AB - Conclusion: The expression of all three subunits of the epithelial sodium channel (ENaC) decreased after the lipopolysaccharide (LPS) injection and normalized as the fluid collection resolved. This implies that fluid collection in acute otitis media may be caused by the inhibition of ENaC function. Objectives: We investigated the expression levels of subunits of ENaC after an injection of LPS into the middle ear cavity of rats. Methods: Seventy-two Sprague-Dawley rats were assigned randomly into the five groups that received an LPS injection and the control group. A transtympanic injection of LPS (1 mg/ml) was done and rats were sacrificed 6, 12, 24, 72, and 120 h after the procedure. Real-time RT-PCR was carried out for ENaC-α, -β, -γ, and immunohistochemistry was performed for ENaC-α and -β. Results: The level of ENaC-α expression decreased 0.50-fold and 0.55-fold at 6 and 12 h, respectively (p < 0.05), but it normalized at 24 h. It increased 3.64-fold at 72 h and 3.24-fold at 120 h (p < 0.05). At 6 and 12 h after the LPS injection into the middle ear cavity, inflammation was induced and ENaC-α immunoreactivity decreased. At 24 h, ENaC-α immunoreactivity was normalized, then at 72 and 120 h after the injection it was increased.
KW - Epithelial sodium channel
KW - Inflammatory response
KW - Lipopolysaccharide
KW - Otitis media
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica