HDAC Antibody Sampler Kit
Related Protocols
Histone deacetylase 4, HD4, Histone deacetylase 1, HD1, RPD3, GON-10, RPD3L1, Histone Deacetylase 2, YY1-Associated Factor 1, HD2, YAF1, Histone deacetylase 3, HD3, SMAP45, RPD3-2, Histone deacetylase 5 HD5, Antigen NY-CO-9, KIAA0600
Total lysates (15 µg protein) from Jurkat (lane 1) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-HDAC1 antibody (clone 1A6C37). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: MW ladder.
Total lysates (15 µg protein) from HepG2 (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-HDAC2 antibody (clone 13G8C67). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) for anti-HDAC2 (upper) or HRP donkey anti-rabbit IgG antibody (clone Poly4064) for anti-GAPDH (lower) and chemiluminescence detection. Purified anti-GAPDH antibody (clone Poly6314) was used as a loading control. Lane M: MW ladder.
Total lysates (15 µg protein) from HeLa (lane 3) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-HDAC3 antibody (clone W15097A). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) for anti-HDAC3 (upper) or HRP donkey anti-rabbit IgG antibody (clone Poly4064) for anti-GAPDH (lower) and chemiluminescence detection. Purified anti-GAPDH antibody (clone Poly6314) was used as a loading control (lower). Lane M: MW ladder. Lane M*: long exposure MW ladder.
Total lysates (15 µg protein) from Jurkat (lane 1) and human platelet (lane 4, negative control) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-HDAC4 antibody (clone 14A9C47, upper). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: MW ladder.
Total lysates (15 µg protein) from Jurkat (lane 1), 293T (lane 5) and NIH3T3 (lane 6) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) purified anti-HDAC5 antibody (clone W15202A, upper). Proteins were visualized using an HRP goat anti-rat IgG secondary antibody (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: MW ladder.
Quantity Avail.
699901 1 kit 268€
The HDAC Antibody Sampler Kit provides a convenient resource to detect HDAC family proteins by Western Blot.
HDAC1 operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. It has been reported to participate in the regulation of eukaryotic gene expression and cell cycle progression. HDAC2 modulates the chromatin structure by removing acetyl groups from the side chain of lysine residues on the N-terminal region of core histones. HDAC2 is highly homologous to HDAC1 in regulating cell proliferation, differentiation and apoptosis. HDAC3 is responsible for the deacetylation of lysine residues on core histones. The deacetylation of histones is important for transcriptional regulation, cell cycle progression, and other developmental related functions. HDAC4 is a member of the class II histone deacetylase family. HDAC4 has been shown to play a crucial role in skeletal formation. HDAC5 is a member of class IIa HDACs, which contain N-terminal extensions with 14-3-3 binding sites. HDAC5 has been suggested to participate in various cellular activities, such as cell proliferation, cell cycle progression, apoptosis, neuronal repair, synoviocyte activation, and myoblast differentiation.
For additional antigen details, please refer to individual product datasheets.
Specificity Clone Size Reactivity Isotype MW (kD)
Anti-HDAC1 1A6C37 25 µg Human, Mouse Mouse IgG1, κ 65
Anti-HDAC2 13G8C67 25 µg Human, Mouse Mouse IgG1, κ 55
Anti-HDAC3 W15097A 25 µg Human, Mouse Mouse IgG2b, κ 50
Anti-HDAC4 14A9C47 25 µg Human Mouse IgG2b, κ 119
Anti-HDAC5 W15202A 25 µg Human, Mouse Rat IgG2b, κ 122
* For detailed information about each specificity, please follow the provided links.
Please refer to individual product datasheets for details.
All antibodies in this kit were purified by affinity chromatography.
All antibodies in the kit are at 0.5mg/ml
Upon receipt, store undiluted at 2-8°C.
WB - Quality tested
Each lot of antibodies in this kit is quality control tested by Western Blotting. For Western blotting, the suggested uses of these reagents are as follows:
Anti-HDAC1: 1:500-1:2000 (0.25-1 µg/mL)
Anti-HDAC2: 1:200-1:1000 (0.5-2.5 µg/mL)
Anti-HDAC3: 1:250-1:1000 (0.5-2 µg/mL)
It is recommended that the reagent be titrated for optimal performance for each application.
For validated or reported applications for these antibodies, please see individual product datasheets.
HDAC4 has 1084 amino acids with a predicted molecular weight of 119 kD. It migrates at 140 kD in SDS-PAGE electrophoresis.
The antibodies do not cross react with other members within HDAC family based on sequence homology and immunogen sequences.
Antigen References
1. Tong J, et al. 1998. Nature 395:917.
2. Grunstein M. 1997. Nature 389:349.
3. Brunmeir R, et al. 2009. Int. J. Dev. Biol. 53:275.
4. Guan JS, et al. 2009. Nature 459:55.
5. Sun Z, et al. 2013. Mol. Cell. 52:769.
6. Sun Z, et al. 2012. Nat. Med. 18:934.
7. Lee DY, et al. 1993. Cell 72:73.
8. Wang AH, et al. 2001. Mol. Cell Biol. 21:5992.
9. Xiao H, et al. 2016. Int. J. Cancer 138:2477.
10. Chen S, et al. 2015. Cell Cycle 14:2520.
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Western Blotting Protocol
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