Experimenter effects on behavioral test scores of eight inbred mouse strains under the influence of ethanol
Martin Bohlen, Erika R. Hayes, Benjamin Bohlen, Jeremy D. Bailoo, John C. Crabbe, Douglas Wahlsten
Civil Environ Construct Engineering
Eight standard inbred mouse strains were evaluated for ethanol effects on a refined battery of behavioral tests in a study that was originally designed to assess the influence of rat odors in the colony on mouse behaviors. As part of the design of the study, two experimenters conducted the tests, and the study was carefully balanced so that equal numbers of mice in all groups and times of day were tested by each experimenter. A defect in airflow in the facility compromised the odor manipulation, and in fact the different odor exposure groups did not differ in their behaviors. The two experimenters, however, obtained markedly different results for three of the tests. Certain of the experimenter effects arose from the way they judged behaviors that were not automated and had to be rated by the experimenter, such as slips on the balance beam. Others were not evident prior to ethanol injection but had a major influence after the injection. For several measures, the experimenter effects were notably different for different inbred strains. Methods to evaluate and reduce the impact of experimenter effects in future research are discussed.
Accelerating rotarod
Motor coordination
Open field activity
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Inbred Strains Mice Medicine & Life Sciences 100%
Behavior Rating Scale Medicine & Life Sciences 93%
Odorants Medicine & Life Sciences 84%
Injections Medicine & Life Sciences 30%
Bohlen, M., Hayes, E. R., Bohlen, B., Bailoo, J. D., Crabbe, J. C., & Wahlsten, D. (2014). Experimenter effects on behavioral test scores of eight inbred mouse strains under the influence of ethanol. Behavioural Brain Research, 272, 46-54. https://doi.org/10.1016/j.bbr.2014.06.017
Bohlen, Martin ; Hayes, Erika R. ; Bohlen, Benjamin et al. / Experimenter effects on behavioral test scores of eight inbred mouse strains under the influence of ethanol. In: Behavioural Brain Research. 2014 ; Vol. 272. pp. 46-54.
@article{edcbb3a15f6740dd9a5c8c28201d1d1c,
title = "Experimenter effects on behavioral test scores of eight inbred mouse strains under the influence of ethanol",
abstract = "Eight standard inbred mouse strains were evaluated for ethanol effects on a refined battery of behavioral tests in a study that was originally designed to assess the influence of rat odors in the colony on mouse behaviors. As part of the design of the study, two experimenters conducted the tests, and the study was carefully balanced so that equal numbers of mice in all groups and times of day were tested by each experimenter. A defect in airflow in the facility compromised the odor manipulation, and in fact the different odor exposure groups did not differ in their behaviors. The two experimenters, however, obtained markedly different results for three of the tests. Certain of the experimenter effects arose from the way they judged behaviors that were not automated and had to be rated by the experimenter, such as slips on the balance beam. Others were not evident prior to ethanol injection but had a major influence after the injection. For several measures, the experimenter effects were notably different for different inbred strains. Methods to evaluate and reduce the impact of experimenter effects in future research are discussed.",
keywords = "Accelerating rotarod, Balance beam, Grip strength, Laboratory environment, Motor coordination, Open field activity",
author = "Martin Bohlen and Hayes, {Erika R.} and Benjamin Bohlen and Bailoo, {Jeremy D.} and Crabbe, {John C.} and Douglas Wahlsten",
note = "Funding Information: Research reported in this paper was supported by grant AA12714 from the National Institute of Alcoholism and Alcohol Abuse . JC also received support from grants AA13519 , AA10760 , and a grant from the US Department of Veterans Affairs . The sponsoring agencies were not involved in the planning, execution or analysis of the experiments and did not contribute to writing the report.",
Bohlen, M, Hayes, ER, Bohlen, B, Bailoo, JD, Crabbe, JC & Wahlsten, D 2014, 'Experimenter effects on behavioral test scores of eight inbred mouse strains under the influence of ethanol', Behavioural Brain Research, vol. 272, pp. 46-54. https://doi.org/10.1016/j.bbr.2014.06.017
Experimenter effects on behavioral test scores of eight inbred mouse strains under the influence of ethanol. / Bohlen, Martin; Hayes, Erika R.; Bohlen, Benjamin et al.
In: Behavioural Brain Research, Vol. 272, 01.10.2014, p. 46-54.
T1 - Experimenter effects on behavioral test scores of eight inbred mouse strains under the influence of ethanol
AU - Bohlen, Martin
AU - Hayes, Erika R.
AU - Bohlen, Benjamin
AU - Bailoo, Jeremy D.
AU - Crabbe, John C.
AU - Wahlsten, Douglas
N1 - Funding Information: Research reported in this paper was supported by grant AA12714 from the National Institute of Alcoholism and Alcohol Abuse . JC also received support from grants AA13519 , AA10760 , and a grant from the US Department of Veterans Affairs . The sponsoring agencies were not involved in the planning, execution or analysis of the experiments and did not contribute to writing the report.
N2 - Eight standard inbred mouse strains were evaluated for ethanol effects on a refined battery of behavioral tests in a study that was originally designed to assess the influence of rat odors in the colony on mouse behaviors. As part of the design of the study, two experimenters conducted the tests, and the study was carefully balanced so that equal numbers of mice in all groups and times of day were tested by each experimenter. A defect in airflow in the facility compromised the odor manipulation, and in fact the different odor exposure groups did not differ in their behaviors. The two experimenters, however, obtained markedly different results for three of the tests. Certain of the experimenter effects arose from the way they judged behaviors that were not automated and had to be rated by the experimenter, such as slips on the balance beam. Others were not evident prior to ethanol injection but had a major influence after the injection. For several measures, the experimenter effects were notably different for different inbred strains. Methods to evaluate and reduce the impact of experimenter effects in future research are discussed.
AB - Eight standard inbred mouse strains were evaluated for ethanol effects on a refined battery of behavioral tests in a study that was originally designed to assess the influence of rat odors in the colony on mouse behaviors. As part of the design of the study, two experimenters conducted the tests, and the study was carefully balanced so that equal numbers of mice in all groups and times of day were tested by each experimenter. A defect in airflow in the facility compromised the odor manipulation, and in fact the different odor exposure groups did not differ in their behaviors. The two experimenters, however, obtained markedly different results for three of the tests. Certain of the experimenter effects arose from the way they judged behaviors that were not automated and had to be rated by the experimenter, such as slips on the balance beam. Others were not evident prior to ethanol injection but had a major influence after the injection. For several measures, the experimenter effects were notably different for different inbred strains. Methods to evaluate and reduce the impact of experimenter effects in future research are discussed.
KW - Accelerating rotarod
KW - Balance beam
KW - Grip strength
KW - Laboratory environment
KW - Motor coordination
KW - Open field activity
Bohlen M, Hayes ER, Bohlen B, Bailoo JD, Crabbe JC, Wahlsten D. Experimenter effects on behavioral test scores of eight inbred mouse strains under the influence of ethanol. Behavioural Brain Research. 2014 Oct 1;272:46-54. doi: 10.1016/j.bbr.2014.06.017