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Title: Lab on a stick: Multi-analyte cellular assays in a microfluidic dipstick
Authors: Reis, Nuno M.
Pivetal, Jeremy
Loo-Zazueta, Ana L.
Barros, Joao M. S.
Edwards, Alexander D.
Publisher: © The Authors. Published by the Royal Society of Chemistry.
Citation: REIS, N.M. ...et al., 2016. Lab on a stick: Multi-analyte cellular assays in a microfluidic dipstick. Lab on a Chip - Miniaturisation for Chemistry and Biology, 16 (15), pp. 2891-2899.
Abstract: © The Royal Society of Chemistry 2016.A new microfluidic concept for multi-analyte testing in a dipstick format is presented, termed "Lab-on-a-Stick", that combines the simplicity of dipstick tests with the high performance of microfluidic devices. Lab-on-a-stick tests are ideally suited to analysis of particulate samples such as mammalian or bacterial cells, and capable of performing multiple different parallel microfluidic assays when dipped into a single sample with results recorded optically. The utility of this new diagnostics format was demonstrated by performing three types of multiplex cellular assays that are challenging to perform in conventional dipsticks: 1) instantaneous ABO blood typing; 2) microbial identification; and 3) antibiotic minimum inhibitory (MIC) concentration measurement. A pressure balance model closely predicted the superficial flow velocities in individual capillaries, that were overestimated by up to one order of magnitude by the Lucas-Washburn equation conventionally used for wicking in cylindrical pores. Lab-on-a-stick provides a cost-effective, simple, portable and flexible multiplex platform for a range of assays, and will deliver a new generation of advanced yet affordable point-of-care tests for global diagnostics.
Description: This is an Open Access Article. It is published by Royal Society of Chemistry under the Creative Commons Attribution 3.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/
Sponsor: This work was funded by EPSRC (grant EP/L013983/1).
DOI: 10.1039/c6lc00332j
Publisher Link: http://dx.doi.org/10.1039/c6lc00332j
Appears in Collections: Published Articles (Chemical Engineering)
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