Universidade Federal do Rio de Janeiro. Programa de Biologia Estrutural. Instituto de Bioquímica Médica e Centro Nacional de Ressonância Magnética Nuclear de Macromoléculas Jiri Jonas. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Programa de Biologia Estrutural. Instituto de Bioquímica Médica e Centro Nacional de Ressonância Magnética Nuclear de Macromoléculas Jiri Jonas, Rio de Janeiro, RJ, Brasil.
Abstract: Viruses are a structurally diverse group of infectious agents that differ widely in their sensitivities to high hydrostatic pressure (HHP). Studies on picornaviruses have demonstrated that these viruses are extremely resistant to HHP treatments, with poliovirus appearing to be the most resistant. Here, the three attenuated poliovirus serotypes were compared with regard to pressure and thermal resistance. We found that HHP does not inactivate any of the three serotypes studied (1–3). Rather, HHP treatment was found to stabilize poliovirus by increasing viral thermal resistance at 37 °C. Identification of new methods that stabilize poliovirus against heat inactivation would aid in the design of a more heat-stable vaccine, circumventing the problems associated with refrigeration during storage and transport of the vaccine prior to use.
Citation: FERREIRA, Evanilce et al. Effects of hydrostatic pressure on the stability and thermostability of poliovirus: A new method for vaccine preservation. Vaccine, Amsterdam, v. 27, n. 9, p. 5332-5337, 2009.