Use of a stainless steel washer platform to study Acinetobacter baumannii adhesion and biofilm formation on abiotic surfaces
Samantha J. Orsinger-Jacobsen, Shenan S. Patel, Ernestine M. Vellozzi, Phillip Gialanella, Leonardo Nimrichter, Kildare Miranda, Luis R. Martinez
increased in incidence as the causative agent of severe disease in troops wounded in Afghanistan and Iraq. Clinical approaches are limited since A. baumannii strains isolated from patients are extremely resistant to current antimicrobials. A. baumannii can survive desiccation and during outbreaks has been recovered from various sites in the patients' environment. To better understand its prevalence in hospital settings, we used a stainless steel washer (SSW) platform to investigate A. baumannii biofilm formation on abiotic surfaces. Scanning electron microscopy demonstrated that A. baumannii forms strong biofilms on stainless steel surfaces. This platform was combined with a colorimetric 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5- [(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay to observe the metabolic activity of bacterial cells, and to facilitate the manipulation and comparison of multiple A. baumannii clinical strains. A strong correlation between XTT and c.f.u. assays was demonstrated. To complement the cell viability assays, A. baumannii biofilm mass was measured by crystal violet staining. Furthermore, the effect of commonly used disinfectants and environmental stressors on A. baumannii biofilms and planktonic cells was compared and characterized. Biofilms on SSWs were significantly more resistant than their planktonic counterparts, providing additional evidence that may allow us to understand the high prevalence of this microbe in hospital settings. Our results validate that SSWs are a simple, versatile and innovative method to study A. baumannii biofilms in vitro.
Microbiology (United Kingdom)
https://doi.org/10.1099/mic.0.068825-0
10.1099/mic.0.068825-0
Dive into the research topics of 'Use of a stainless steel washer platform to study Acinetobacter baumannii adhesion and biofilm formation on abiotic surfaces'. Together they form a unique fingerprint.
Acinetobacter baumannii Medicine & Life Sciences 100%
Stainless Steel Medicine & Life Sciences 95%
Biofilms Medicine & Life Sciences 80%
2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide Medicine & Life Sciences 17%
Desiccation Medicine & Life Sciences 15%
Gentian Violet Medicine & Life Sciences 13%
Afghanistan Medicine & Life Sciences 13%
Iraq Medicine & Life Sciences 12%
Orsinger-Jacobsen, S. J., Patel, S. S., Vellozzi, E. M., Gialanella, P., Nimrichter, L., Miranda, K., & Martinez, L. R. (2013). Use of a stainless steel washer platform to study Acinetobacter baumannii adhesion and biofilm formation on abiotic surfaces. Microbiology (United Kingdom), 159(PART 12), 2594-2604. https://doi.org/10.1099/mic.0.068825-0
Use of a stainless steel washer platform to study Acinetobacter baumannii adhesion and biofilm formation on abiotic surfaces. / Orsinger-Jacobsen, Samantha J.; Patel, Shenan S.; Vellozzi, Ernestine M. et al.
In: Microbiology (United Kingdom), Vol. 159, No. PART 12, 12.2013, p. 2594-2604.
Orsinger-Jacobsen, SJ, Patel, SS, Vellozzi, EM, Gialanella, P, Nimrichter, L, Miranda, K & Martinez, LR 2013, 'Use of a stainless steel washer platform to study Acinetobacter baumannii adhesion and biofilm formation on abiotic surfaces', Microbiology (United Kingdom), vol. 159, no. PART 12, pp. 2594-2604. https://doi.org/10.1099/mic.0.068825-0
Orsinger-Jacobsen SJ, Patel SS, Vellozzi EM, Gialanella P, Nimrichter L, Miranda K et al. Use of a stainless steel washer platform to study Acinetobacter baumannii adhesion and biofilm formation on abiotic surfaces. Microbiology (United Kingdom). 2013 Dec;159(PART 12):2594-2604. doi: 10.1099/mic.0.068825-0
Orsinger-Jacobsen, Samantha J. ; Patel, Shenan S. ; Vellozzi, Ernestine M. et al. / Use of a stainless steel washer platform to study Acinetobacter baumannii adhesion and biofilm formation on abiotic surfaces. In: Microbiology (United Kingdom). 2013 ; Vol. 159, No. PART 12. pp. 2594-2604.
@article{1c2a54f32d79476f8bb1cbd56a28b313,
title = "Use of a stainless steel washer platform to study Acinetobacter baumannii adhesion and biofilm formation on abiotic surfaces",
abstract = "increased in incidence as the causative agent of severe disease in troops wounded in Afghanistan and Iraq. Clinical approaches are limited since A. baumannii strains isolated from patients are extremely resistant to current antimicrobials. A. baumannii can survive desiccation and during outbreaks has been recovered from various sites in the patients' environment. To better understand its prevalence in hospital settings, we used a stainless steel washer (SSW) platform to investigate A. baumannii biofilm formation on abiotic surfaces. Scanning electron microscopy demonstrated that A. baumannii forms strong biofilms on stainless steel surfaces. This platform was combined with a colorimetric 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5- [(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay to observe the metabolic activity of bacterial cells, and to facilitate the manipulation and comparison of multiple A. baumannii clinical strains. A strong correlation between XTT and c.f.u. assays was demonstrated. To complement the cell viability assays, A. baumannii biofilm mass was measured by crystal violet staining. Furthermore, the effect of commonly used disinfectants and environmental stressors on A. baumannii biofilms and planktonic cells was compared and characterized. Biofilms on SSWs were significantly more resistant than their planktonic counterparts, providing additional evidence that may allow us to understand the high prevalence of this microbe in hospital settings. Our results validate that SSWs are a simple, versatile and innovative method to study A. baumannii biofilms in vitro.",
author = "Orsinger-Jacobsen, {Samantha J.} and Patel, {Shenan S.} and Vellozzi, {Ernestine M.} and Phillip Gialanella and Leonardo Nimrichter and Kildare Miranda and Martinez, {Luis R.}",
doi = "10.1099/mic.0.068825-0",
journal = "Journal of General Microbiology",
publisher = "Society for General Microbiology",
number = "PART 12",
T1 - Use of a stainless steel washer platform to study Acinetobacter baumannii adhesion and biofilm formation on abiotic surfaces
AU - Orsinger-Jacobsen, Samantha J.
AU - Patel, Shenan S.
AU - Vellozzi, Ernestine M.
AU - Gialanella, Phillip
AU - Nimrichter, Leonardo
AU - Miranda, Kildare
AU - Martinez, Luis R.
N2 - increased in incidence as the causative agent of severe disease in troops wounded in Afghanistan and Iraq. Clinical approaches are limited since A. baumannii strains isolated from patients are extremely resistant to current antimicrobials. A. baumannii can survive desiccation and during outbreaks has been recovered from various sites in the patients' environment. To better understand its prevalence in hospital settings, we used a stainless steel washer (SSW) platform to investigate A. baumannii biofilm formation on abiotic surfaces. Scanning electron microscopy demonstrated that A. baumannii forms strong biofilms on stainless steel surfaces. This platform was combined with a colorimetric 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5- [(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay to observe the metabolic activity of bacterial cells, and to facilitate the manipulation and comparison of multiple A. baumannii clinical strains. A strong correlation between XTT and c.f.u. assays was demonstrated. To complement the cell viability assays, A. baumannii biofilm mass was measured by crystal violet staining. Furthermore, the effect of commonly used disinfectants and environmental stressors on A. baumannii biofilms and planktonic cells was compared and characterized. Biofilms on SSWs were significantly more resistant than their planktonic counterparts, providing additional evidence that may allow us to understand the high prevalence of this microbe in hospital settings. Our results validate that SSWs are a simple, versatile and innovative method to study A. baumannii biofilms in vitro.
AB - increased in incidence as the causative agent of severe disease in troops wounded in Afghanistan and Iraq. Clinical approaches are limited since A. baumannii strains isolated from patients are extremely resistant to current antimicrobials. A. baumannii can survive desiccation and during outbreaks has been recovered from various sites in the patients' environment. To better understand its prevalence in hospital settings, we used a stainless steel washer (SSW) platform to investigate A. baumannii biofilm formation on abiotic surfaces. Scanning electron microscopy demonstrated that A. baumannii forms strong biofilms on stainless steel surfaces. This platform was combined with a colorimetric 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5- [(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay to observe the metabolic activity of bacterial cells, and to facilitate the manipulation and comparison of multiple A. baumannii clinical strains. A strong correlation between XTT and c.f.u. assays was demonstrated. To complement the cell viability assays, A. baumannii biofilm mass was measured by crystal violet staining. Furthermore, the effect of commonly used disinfectants and environmental stressors on A. baumannii biofilms and planktonic cells was compared and characterized. Biofilms on SSWs were significantly more resistant than their planktonic counterparts, providing additional evidence that may allow us to understand the high prevalence of this microbe in hospital settings. Our results validate that SSWs are a simple, versatile and innovative method to study A. baumannii biofilms in vitro.
U2 - 10.1099/mic.0.068825-0
DO - 10.1099/mic.0.068825-0
JO - Journal of General Microbiology
JF - Journal of General Microbiology
IS - PART 12