Analysis of signaling pathways activated by CCN proteins
Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa
Graduate School of Medicine Dentistry and Pharmaceutical Sciences
Dental School
Academic Field of Medicine, Dentistry and Pharmaceutical Sciences
CCN family proteins activate multiple intracellular phosphorylated kinase cascades to yield the multiple physiological functions of a variety of target cells. In this chapter, we describe our protocol examining the effects of these proteins on signal transduction pathways, especially mitogen-activated protein kinase cascades, activated by CCN member proteins, which examinations have been carried out mainly by using Western blotting methodologies.
Humana Press Inc.
https://doi.org/10.1007/978-1-4939-6430-7_14
CCN signaling
ERK1/2 (p44/42 MAPK)
MAPK pathway
PI3K-PKB/Akt pathway
10.1007/978-1-4939-6430-7_14
Dive into the research topics of 'Analysis of signaling pathways activated by CCN proteins'. Together they form a unique fingerprint.
CCN Intercellular Signaling Proteins Medicine & Life Sciences 100%
Signal Transduction Medicine & Life Sciences 43%
Phosphotransferases Medicine & Life Sciences 42%
Kawaki, H., Kubota, S., & Takigawa, M. (2017). Analysis of signaling pathways activated by CCN proteins. In Methods in Molecular Biology (pp. 139-143). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_14
Analysis of signaling pathways activated by CCN proteins. / Kawaki, Harumi; Kubota, Satoshi; Takigawa, Masaharu.
Methods in Molecular Biology. Humana Press Inc., 2017. p. 139-143 (Methods in Molecular Biology; Vol. 1489).
Kawaki, H, Kubota, S & Takigawa, M 2017, Analysis of signaling pathways activated by CCN proteins. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1489, Humana Press Inc., pp. 139-143. https://doi.org/10.1007/978-1-4939-6430-7_14
Kawaki H, Kubota S, Takigawa M. Analysis of signaling pathways activated by CCN proteins. In Methods in Molecular Biology. Humana Press Inc. 2017. p. 139-143. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6430-7_14
Kawaki, Harumi ; Kubota, Satoshi ; Takigawa, Masaharu. / Analysis of signaling pathways activated by CCN proteins. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 139-143 (Methods in Molecular Biology).
@inbook{4c16f3b0658c42c1be8166f596a9316b,
title = "Analysis of signaling pathways activated by CCN proteins",
abstract = "CCN family proteins activate multiple intracellular phosphorylated kinase cascades to yield the multiple physiological functions of a variety of target cells. In this chapter, we describe our protocol examining the effects of these proteins on signal transduction pathways, especially mitogen-activated protein kinase cascades, activated by CCN member proteins, which examinations have been carried out mainly by using Western blotting methodologies.",
keywords = "CCN signaling, ERK1/2 (p44/42 MAPK), JNK, MAPK pathway, PI3K-PKB/Akt pathway, Western blotting, p38 MAPK",
author = "Harumi Kawaki and Satoshi Kubota and Masaharu Takigawa",
note = "Funding Information: This work was supported by JSPS KAKENHI Grant Numbers JP24390415 and JP15H05014 to M.T., JP25462886 to S.K., JP26462984 to H.K., and The Science Research Promotion Fund of The Promotion and Mutual Aid Corporation for Private Schools of Japan to H.K. The authors gratefully thank Ms. Yoshiko Miyake as well for her secretarial assistance. Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",
doi = "10.1007/978-1-4939-6430-7_14",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
booktitle = "Methods in Molecular Biology",
T1 - Analysis of signaling pathways activated by CCN proteins
AU - Kawaki, Harumi
AU - Kubota, Satoshi
AU - Takigawa, Masaharu
N1 - Funding Information: This work was supported by JSPS KAKENHI Grant Numbers JP24390415 and JP15H05014 to M.T., JP25462886 to S.K., JP26462984 to H.K., and The Science Research Promotion Fund of The Promotion and Mutual Aid Corporation for Private Schools of Japan to H.K. The authors gratefully thank Ms. Yoshiko Miyake as well for her secretarial assistance. Publisher Copyright: © Springer Science+Business Media New York 2017. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
N2 - CCN family proteins activate multiple intracellular phosphorylated kinase cascades to yield the multiple physiological functions of a variety of target cells. In this chapter, we describe our protocol examining the effects of these proteins on signal transduction pathways, especially mitogen-activated protein kinase cascades, activated by CCN member proteins, which examinations have been carried out mainly by using Western blotting methodologies.
AB - CCN family proteins activate multiple intracellular phosphorylated kinase cascades to yield the multiple physiological functions of a variety of target cells. In this chapter, we describe our protocol examining the effects of these proteins on signal transduction pathways, especially mitogen-activated protein kinase cascades, activated by CCN member proteins, which examinations have been carried out mainly by using Western blotting methodologies.
KW - CCN signaling
KW - ERK1/2 (p44/42 MAPK)
KW - JNK
KW - MAPK pathway
KW - PI3K-PKB/Akt pathway
KW - Western blotting
KW - p38 MAPK
U2 - 10.1007/978-1-4939-6430-7_14
DO - 10.1007/978-1-4939-6430-7_14
T3 - Methods in Molecular Biology
BT - Methods in Molecular Biology
PB - Humana Press Inc.