pFUSE-CHIg-hE is a cloning plasmid that expresses the constant region of the human IgE heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hE additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.
2015 Mol Cell Proteomics. 14(5):1265-74.
Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright GJ.